LOCUS Exported 809 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION TEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformation. ACCESSION . VERSION . KEYWORDS pCEV-G3-Km SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 809) AUTHORS Vickers CE, Bydder SF, Zhou Y, Nielsen LK TITLE Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae. JOURNAL Microb Cell Fact. 2013 Oct 25;12(1):96. PUBMED 24161108 REFERENCE 2 (bases 1 to 809) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..809 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 202..219 /label=L4440 /note="L4440 vector, forward primer" terminator 291..480 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" CDS complement(510..539) /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" primer_bind complement(556..575) /label=T7 /note="T7 promoter, forward primer" promoter complement(557..575) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 61 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 121 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 181 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 241 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggat cttcgagcgt 301 cccaaaacct tctcaagcaa ggttttcagt ataatgttac atgcgtacac gcgtctgtac 361 agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat aactataaaa 421 aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat 481 cttagctagc cgcggtacca agcttactcg aggtcttctt cggaaatcaa cttctgttcc 541 atgtcgacgc ccgggcccta tagtgagtcg tattacggat ccttgtaatt aaaacttaga 601 ttagattgct atgctttctt tctaatgagc aagaagtaaa aaaagttgta atagaacaag 661 aaaaatgaaa ctgaaacttg agaaattgaa gaccgtttat taacttaaat atcaatggga 721 ggtcatcgaa agagaaaaaa atcaaaaaaa aaaattttca agaaaaagaa acgtgataaa 781 aatttttatt gcctttttcg acgaagaaa //