LOCUS Exported 901 bp ds-DNA linear SYN 13-JUN-2021 DEFINITION This plasmid encode NmCas9 with two NLS and a HA tag. ACCESSION . VERSION . KEYWORDS pSimpleII-NLS-NmCas9-HA-NLS(s) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 901) AUTHORS Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA TITLE Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. JOURNAL Proc Natl Acad Sci U S A. 2013 Aug 12. PUBMED 23940360 REFERENCE 2 (bases 1 to 901) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..901 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 5..14 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 17..37 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 caccgccacc atggtgccta agaagaagag aaaggtggct gccttcaaac ctaattcaat 61 caactacatc ctcggcctcg atatcggcat cgcatccgtc ggctgggcga tggtagaaat 121 tgacgaagaa gaaaacccca tccgcctgat tgatttgggc gtgcgcgtat ttgagcgtgc 181 cgaagtaccg aaaacaggcg actcccttgc catggcaagg cgtttggcgc gcagtgttcg 241 ccgcctgacc cgccgtcgcg cccaccgcct gcttcggacc cgccgcctat tgaaacgcga 301 aggcgtatta caagccgcca attttgacga aaacggcttg attaaatcct taccgaatac 361 accatggcaa cttcgcgcag ccgcattaga ccgcaaactg acgcctttag agtggtcggc 421 agtcttgttg catttaatca aacatcgcgg ctatttatcg caacggaaaa acgagggcga 481 aactgccgat aaggagcttg gcgctttgct taaaggcgta gccggcaatg cccatgcctt 541 acagacaggc gatttccgca caccggccga attggcttta aataaatttg agaaagaaag 601 cggccatatc cgcaatcagc gcagcgatta ttcgcatacg ttcagccgca aagatttaca 661 ggcggagctg attttgctgt ttgaaaaaca aaaagaattt ggcaatccgc atgtttcagg 721 cggccttaaa gaaggtattg aaaccctact gatgacgcaa cgccctgccc tgtccggcga 781 tgccgttcaa aaaatgttgg ggcattgcac cttcgaaccg gcagagccga aagccgctaa 841 aaacacctac acagccgaac gtttcatctg gctgaccaag ctgaacaacc tgcgtatttt 901 a //