LOCUS Exported 4012 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAG76 GLCCI1 3'UTR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4012) AUTHORS Farh KK, Grimson A, Jan C, Lewis BP, Johnston WK, Lim LP, Burge CB, Bartel DP TITLE The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. JOURNAL Science. 2005 Dec 16. 310(5755):1817-21. PUBMED 16308420 REFERENCE 2 (bases 1 to 4012) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4012 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 29..150 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(40..59) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 94..113 /label=SV40pA-R /note="SV40 polyA, reverse primer" promoter 283..387 /gene="bla" /label=AmpR promoter CDS 388..1248 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(606..625) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1419..2007 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1908..1927 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 2136..2493 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2159..2179) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2344..2479 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2406..2425 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 2563..2695 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 2678..2697 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 2740..2758 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2768..3703 /codon_start=1 /product="luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)" /label=Rluc /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" ORIGIN 1 ctagagcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca 61 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt 121 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt 181 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt 241 aaaatcgata aggatccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 601 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 901 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 1021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 1081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 1141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 1201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 1261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 1321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 1381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 1441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 1501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 1561 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 1621 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 1681 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 1741 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 1801 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 1861 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 1921 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 1981 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 2041 gccttttgct ggccttttgc tcacatggct cgacagatct gcgcagcacc atggcctgaa 2101 ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg 2161 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 2221 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 2281 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 2341 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 2401 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 2461 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg attcttctga cacaacagtc 2521 tcgaacttaa gctgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca 2581 agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt 2641 ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc 2701 cactcccagt tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct 2761 agccaccatg acttcgaaag tttatgatcc agaacaaagg aaacggatga taactggtcc 2821 gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta attattatga 2881 ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg cctcttctta 2941 tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta taccagacct 3001 tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac ttgatcatta 3061 caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca tttttgtcgg 3121 ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag ataagatcaa 3181 agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg aatggcctga 3241 tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg ttttggagaa 3301 taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag aaccagaaga 3361 atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc caacattatc 3421 atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac aaattgttag 3481 gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta ttgaatcgga 3541 cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta atactgaatt 3601 tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa tgggaaaata 3661 tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg agctctattt 3721 tatgcatttc cctttcctca ttacattcca cattcttaga ataagaagtg cattcaatcc 3781 taggagaatg ataatcctgg acatgggtga acatgaggag aaccagcaaa atctgtggtg 3841 tttgacatca ctttgtcatg tggttacaag taaaacaact gttgcattca ctgtttcaac 3901 atgtgtacat gtggcttttt taaaagttca ggtgttgctc agtaaaggac tgtgacaatg 3961 ttgcaaataa agtgttcagt actggactgt acataaacat tccacattgt gt //