LOCUS       Exported                 890 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pThy1-Autobow
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      650..659
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    complement(675..708)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     polyA_signal    745..866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(756..775)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     810..829
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gccacctcgt gctgctcgac gtaggtctct ttgtcggcct ccttgattct ttccagtctt
       61 ctgtccacat agtagacgcc gggcatcttg aggttcttag cgggtttctt ggatctgtat
      121 gtggtcttca agttgcagat caggtggccc ccgcccacga gcttcagggc catgtcggct
      181 ctgccttcca ggccgccgtc agcggggtac agggtctcgg tggaggcctc ccagccgagt
      241 gttttcttct gcatcacagg gccgttggat gggaagttca cccctctgat cttgacgttg
      301 tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac gcccccgtct
      361 tcgtatgtgg tgactctctc ccatgtgaag ccctcgggga aggactgctt aaagaagtcg
      421 gggatgccct gggtgtggtt gatgaaggtt ttgctgccgt acatgaagct ggtagccagg
      481 atgtcgaagg cgaaggggag agggccgccc tcgaccgcct tgattctcat ggtctgggtg
      541 ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtggtggtt gttcacggtg
      601 ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcgctcac catggtggca
      661 gtactggata cttgataact tcgtataagg tatactatac gaagttatat gaagaacgcg
      721 aagattatga agctcttcac gccttaagat acattgatga gtttggacaa accacaacta
      781 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      841 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
//
