LOCUS Exported 3908 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION full length coding sequence for zebrafish fli1a gene in gateway middle entry vector. ACCESSION . VERSION . KEYWORDS 375 pME-fli1a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3908) AUTHORS Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N TITLE Post-transcriptional mechanisms contribute to Etv2 repression during vascular development. JOURNAL Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 10.1016/j.ydbio.2013.08.028. PUBMED 24036310 REFERENCE 2 (bases 1 to 3908) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3908 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2033..2132) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2149..2168) /label=T7 /note="T7 promoter, forward primer" promoter complement(2150..2168) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2173..2189) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2173..2189) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2291..2310) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2302..3111 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2369..2388) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3258..3846 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3747..3766 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggcttg cgcaaaatgg acggaactat taaggaggcg ctgtcagtgg tgagtgaaga 721 ccagtctctg ttcgagcctc catacgccgc tgctgctcct ttacccaaga cagacatgac 781 tgcgtccggg actcaggatt acggccagac gcacaaaatc aaccccattc cccctcagca 841 agagtggatt aatcagccgg tgcgggtgaa cgtcaagcga gagtatgacc acatcaacgg 901 atccagagag tcgccggtgg actgcagcgt ggggaaatgc aataaaatgg tgggcgggac 961 cgaagcgtct cagatgaact atactggcta catggatgag aaatgtgctc cgcctccaaa 1021 catgacgacc aatgagagga gagtcatcgt ccccgcagac ccgtctctgt ggtctccgga 1081 tcacgtgcgg cagtggttag actgggccat taaggagtac ggtcttcagg agatcgacac 1141 ggccatgttt cacagcacag atggaaaaga gctctgcaag atgagcaaag acgacttcct 1201 gagactcacc agcgtttata acaccgaggt ccttctctca catctcaatt acctcaggga 1261 aagtagctca tcgatatcat acaacacgcc atctcacgct gaccagtccc ctcgcctggc 1321 tgcaaaagac gatgcgtctt atgatgctgt acggaggacg ggctggtcga acaacatgca 1381 cagcggaaaa ggctctccaa cagtggtctc tcagagcgtg tccaaaaacc cggatcagcc 1441 tcgtcctcag ccagatcctt atcagatatt aggacccacc agcagtcgac ttgctaaccc 1501 aggctcaggt cagatccagc tgtggcagtt cctgctggag ctcctgtccg acagcgccaa 1561 cgccggctgc atcacctggg aaggaaccaa cggagagttc aagatgacgg acccagacga 1621 ggtggcacgg cgctggggcg aacgcaagag caaacccaac atgaactacg acaagctgag 1681 ccgagccctg cgctactact acgacaagaa catcatgacc aaagtgcacg gcaagcgcta 1741 cgcctacaag ttcgacttcc acggcatcgc ccaggccctg caaccccatc ccaccgagtc 1801 caccatgtac aagtacccaa gcgagctgcc gtacgtgcct agctatcacg cccaccagca 1861 gaaggtcaac ttcgtctctc ctcaccctcc gtcaatgccc gtcacctcct ccaacttctt 1921 cggtcccaca accccatact ggagctcccc accaggaggt atttacccca atcccagcgt 1981 cccacgccat gccaattcac acgtgccttc acaccttggt agttactact aaacccagct 2041 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag 2101 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag 2161 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga 2221 tgttacattg cacaagataa aataatatca tcatgaacaa taaaactgtc tgcttacata 2281 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta 2341 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa 2401 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa 2461 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg 2521 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg 2581 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat 2641 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct 2701 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga 2761 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt 2821 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact 2881 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt 2941 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc 3001 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat 3061 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg 3121 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca 3181 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa 3241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 3301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 3361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 3421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 3481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 3541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 3601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 3661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 3721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 3781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 3841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 3901 cacatgtt //