LOCUS       Exported                3160 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  full length coding sequence for zebrafish lin28 gene in gateway 
            middle entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    1132 pME-Lin28
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N
  TITLE     Post-transcriptional mechanisms contribute to Etv2 repression during
            vascular development.
  JOURNAL   Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028.
  PUBMED    24036310
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1287..1386)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1403..1422)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1404..1422)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1427..1443)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1427..1443)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1545..1564)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1556..2365
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1623..1642)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2512..3100
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3001..3020
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgc gccaccatgc ccccggcaaa tccgcatctc aatcacacag gtggatgtac
      721 aaagacagag gaggaagaag ccgcgagctc cgaggaagac tcaggctctt ttcacggctc
      781 tggggtctgc aaatggttta acgtgcggat gggcttcgga tttctgtcca tgacccaccg
      841 ggagggcatc tgtctggact cacccgtgga tgtcttcgtc catcagagca agcttcatat
      901 ggagggtttt cgcagtctga aggaaggaga agcagtggaa ttcaccttta aaagatcctc
      961 taagggtctg gagtccttac aagtgacggg gcctggaggg gcaccatgtg tgggcagcga
     1021 gaagaaacct aagggtaccc aaaagaggcg gtcaaaagga gatcgatgct tcaactgtgg
     1081 aggacccaat catcatgcca aagaatgcca gctgccacct cagccgaaaa agtgccattt
     1141 ttgccaaagc atctcccaca tggtggccaa ctgcccaatc aaagcacagc agttgtcacc
     1201 aggatctcag ggcaaatcga caacctcaac aggagaagag gaggacatga gccacactcc
     1261 cttactgcca gagagcactg attaggaccc agctttcttg tacaaagttg gcattataag
     1321 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     1381 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     1441 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaataat
     1501 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     1561 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     1621 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     1681 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     1741 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     1801 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     1861 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     1921 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     1981 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     2041 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     2101 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     2161 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     2221 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     2281 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     2341 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     2401 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     2461 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2521 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2581 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2641 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2701 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2761 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2821 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2881 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2941 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3001 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3061 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3121 tttacggttc ctggcctttt gctggccttt tgctcacatg
//
