LOCUS       Exported                1035 bp ds-DNA     linear   SYN 15-JUN-2021
DEFINITION  LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. 
            Binds to CIB1 upon blue light stimulation. Synapsin promoter for 
            neuronal expression. See LITE2.0 for optimized LITE activators..
ACCESSION   .
VERSION     .
KEYWORDS    LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(35..88)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(110..259)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(284..304)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccaccccggt gaacagctcc tcgcccttgc tcactgggcc aggattctcc tcgacgtcac
       61 cgcatgttag cagacttcct ctgccctctc cactgcctct agagttaatc agcatgtcca
      121 ggtcgaaatc atcaagggcg tcactgccga gcatgtcgag gtcaaagtca tcaagggcat
      181 ccgaaccaag catgtcaagg tcaaaatcat cgagggcgtc acttcccagc atatccagat
      241 caaaatcgtc caatgcgtca gcccgtccgg aaccgctggc ctccaccttt ctcttcttct
      301 tggggctagc cggcgctgct ccgatcataa tctgcgcttc gcgtgttctt gagatggctt
      361 tcgccagcaa ttctcgtgcg gtgtcaatat cgacgatggg tttcgcgtaa ttcgtcccca
      421 attcgacccc tgaggcttta aggacggtca ggggagcatc ccacgggtgg tgaatccact
      481 cggtgggcag acgggccagc tctgggagcc actgcctgat gtattctccc tccgggtcgt
      541 actttgctcc ttgcagtgca gggttatcca atcggtcaag ttcatgcccg tcagggatgg
      601 aacccgaaat gtactgccac ccaagaatat cgcactccaa gtccgcatcc agaagcgtgt
      661 cccaaaagta tttcatcccc catttccagg gcaacaagag gaacttaacg gcaaagctac
      721 taacgatgac tcgaatacgg ttgtgcatcc aacccgttgc ccacagctct ctcatacccg
      781 catccaccaa cgggtatccc gtgcgcccct gccgccaggc cttgaatttg tccacgtccg
      841 cgtcccacgg aaagaagcgg aggtggctga ggagggattg ttcatgtgta aacggaaagt
      901 tgaagcagat gtatcgtgaa tactccctaa ggccgattcc tctcaaaaac aaatcggcag
      961 actcctctcc ttcagagttt ttgtcgcgag cccaaatgat ttgcttcatc cgcgcacatt
     1021 ggaacacatg cctca
//
