LOCUS Exported 9795 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses human NKCC1 mutant lacking cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.. ACCESSION . VERSION . KEYWORDS pcDNA3.1 Flag YFP hNKCC1 cysteineless (NT868) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9795) AUTHORS Somasekharan S, Monette MY, Forbush B TITLE Functional Expression of Human NKCC1 from a Synthetic Cassette-Based cDNA: Introduction of Extracellular Epitope Tags and Removal of Cysteines. JOURNAL PLoS One. 2013 Dec 5;8(12):e82060. doi: 10.1371/journal.pone.0082060. PUBMED 24339991 REFERENCE 2 (bases 1 to 9795) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9795 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 19..28 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 31..96 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 112..822 /codon_start=1 /product="enhanced variant of YFP (Heikal et al., 2001)" /label=Citrine /note="mammalian codon-optimized" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEF VTAAGITLGMDELYK" CDS 112..822 /codon_start=1 /product="enhanced YFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEYFP /note="mammalian codon-optimized" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEF VTAAGITLGMDELYK" primer_bind complement(151..172) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(412..431) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 759..780 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(4495..4512) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4501..4725 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4771..5199 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4858..4877) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5068..5089 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5208..5228) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5213..5542 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5393..5528 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5455..5474 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5609..6403 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5663..5682) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6273..6292 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 6577..6698 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6614..6633) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6668..6687 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6747..6763) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6747..6763) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6760..6782) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6771..6787 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6795..6825) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6840..6861 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6978..6995) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7149..7734) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7229..7248) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7905..8765) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8528..8547 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8766..8870) /gene="bla" /label=AmpR promoter primer_bind complement(8945..8964) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 9136..9515 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 9516..9719 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 9670..9690 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 9764..9783 /label=T7 /note="T7 promoter, forward primer" promoter 9764..9782 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 gctagcccgg gcacggccgc caccatggga gactacaaag accatgacgg tgattataaa 61 gatcacgaca tcgactacaa ggatgacgat gacaagggtg accctaccgt cagcaagggc 121 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 181 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 241 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc 301 ggctacggcc tgatgtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc 361 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 421 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 481 ctgaagggca tcgacttcaa ggaggacggc aacatcctag ggcacaagct ggagtacaac 541 tacaacagcc acaacgttta tatcatggcc gacaagcaga agaacggcat caaggtgaac 601 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 661 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag 721 tccaaactga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 781 accgccgccg ggatcactct cggcatggac gagctctaca agggtgacct ggagccgagg 841 cccacggctc ctagctccgg cgcgccaggc ctcgccggag tcggcgaaac gccgagcgcc 901 gctgcgctgg ccgcagccag ggtggaactg cccggaaccg ctgtgccctc ggtgccggag 961 gatgctgcgc ccgcgagccg ggacgggggc ggggtccgcg atgagggacc agccgccgcc 1021 ggtgacggcc tgggcagacc cttggggcct accccgagcc agagccgttt ccaggtggac 1081 ctcgtttccg agaacgcagg gcgggctgct gctgcggcgg cggcggctgc tgcggccgcc 1141 gccgctgctg gtgccggggc gggggccaag cagactcctg ccgacgggga agccagcggc 1201 gagagcgagc cagctaaagg cagcgaggaa gccaaaggcc gcttccgcgt taacttcgta 1261 gatcctgctg ccagctcgtc cgcagaagat agcctgtcag atgccgccgg ggtcggagtc 1321 gatggaccca acgtgagctt ccagaacggc ggggacacgg tactgagcga gggcagcagc 1381 ctccattccg gtggcggcgg cggctccggc caccaccagc actactatta tgatacccac 1441 accaacacct actatctgcg caccttcggc cacaacacaa tggacgcggt acccaggatc 1501 gaccactacc ggcacacagc cgcgcagctc ggcgagaagc tgctccggcc tagcctggcc 1561 gagctgcatg acgagctgga aaaggaacct tttgaggatg gctttgcaaa tggggaagaa 1621 agcactccaa cacgtgatgc tgtggtcacg tacacggccg aaagtaaagg agtcgtgaag 1681 tttggctgga tcaagggtgt actggtacgt gccatgttaa acatttgggg tgtgatgctt 1741 ttcattcgtc tctcatggat tgtgggacaa gctggaattg gtctatcagt ccttgtaata 1801 atgatggcta ctgttgtgac aactatcaca ggattgtcca ctagcgccat cgcaactaat 1861 ggatttgtaa ggggaggcgg cgcatattat ttaatatcta gaagtctagg gcctgaattt 1921 ggtggtgcca ttggtctaat cttcgccttt gccaacgctg ttgctgttgc tatgtatgtg 1981 gttggatttg cagaaaccgt ggtggagttg ctgaaagaac attccattct tatgatagat 2041 gaaatcaatg acatccggat tattggagcc attacagtcg tgattctttt aggtatctca 2101 gtagccggta tggagtggga agcaaaagct cagattgttc ttttggtgat cctacttctt 2161 gctattggtg atttcgtcat aggaacattt atcccactcg agagcaagaa gccaaaaggg 2221 ttttttggct ataaatctga aatctttaat gagaactttg gccccgattt tcgagaagaa 2281 gaaactttct tttctgtatt tgccatcttt tttcctgctg caactggaat tctggctggc 2341 gcaaatatct caggtgatct tgcagatcct cagtcagcca tacccaaagg aacactccta 2401 gccattttaa ttactacatt ggtttatgta ggaattgccg tgtctgtagg ttctatggtc 2461 gtacgcgatg ccactggtaa tgttaatgac actatcgtaa cagagctaac aaactctact 2521 agcgcagcct ctaaattaaa ctttgatttt tcatcttctg aatccagtcc ttctagctac 2581 ggcctaatga acaactttca ggtaatgagt atggtctcag gatttacacc actaatttcc 2641 gcgggcatat tctctgccac tctgtcttcc gcattagcat ccctagtgag tgctcccaag 2701 atatttcagg ccctatccaa agacaacatc taccccgctt tccagatgtt tgctaaaggt 2761 tatgggaaaa ataatgagcc attacgtggc tacatcttaa cattcttaat tgcacttgga 2821 ttcatcttaa ttgccgaact gaatgttatt gcaccaatta tctcaaactt cttccttgcc 2881 agctatgccc tcatcaattt ttcagtattc catgcttcac tggcaaaaag cccaggatgg 2941 cgaccggcat ttaaatacta caatatgtgg atctcacttc ttggagcaat tctttccgtg 3001 atagtaatgt tcgtcattaa ctggtgggct gctttgctaa cctacgtgat agtccttggt 3061 ctctatattt atgttaccta caaaaaaccc gatgtgaatt ggggatcctc tacacaagcc 3121 ctgacttacc tcaatgccct gcaacatagt attaggcttt ctggcgtgga agaccatgtg 3181 aaaaacttta ggccacagtc ccttgttatg acaggagcgc caaactcacg gccggcctta 3241 cttcatcttg ttcacgattt cacaaaaaat gttggtttga tgatctctgg ccacgtacac 3301 atgggaccac gcagacaagc catgaaagaa atgtccatcg accaagccaa atatcagcgc 3361 tggcttatta agaacaaaat gaaggctttt tatgcaccag tacatgcaga tgacctcaga 3421 gaaggggccc aatacttgat gcaggctgct ggcctgggcc gtatgaagcc aaacacacta 3481 gtccttggat ttaagaaaga ttggttgcaa gcagatatga gggatgtgga tatgtacata 3541 aacttatttc acgatgcttt tgacatacaa tatggcgtag tggttattcg cctaaaagaa 3601 ggtctggata ttagtcacct gcagggacaa gaagaattat tgtcatcaca agagaaatct 3661 cctggcacca aggatgtcgt agtaagtgtg gaatatagta aaaagtccga tttagatact 3721 tcgaaacccc tcagtgaaaa accaattacc cataaagtcg aggaagaaga tggcaagact 3781 gcaactcaac cactgttgaa aaaagaatcc aaaggcccta ttgtgccttt gaatgtagct 3841 gaccaaaagc ttcttgaagc tagtacacag tttcagaaaa aacaaggaaa gaatactatt 3901 gatgtctggt ggctttttga tgacggaggt ctcacattac tcatacctta ccttctgacg 3961 accaagaaaa aatggaaaga ctctaagatc agagtattca ttggtggaaa gataaacaga 4021 atagaccatg acagaagagc gatggctact ttactgagca agttcagaat cgatttttct 4081 gacatcatgg ttctaggaga catcaatacc aaaccaaaga aagaaaacat tatagctttc 4141 gaggaaatca ttgagccata cagacttcac gaagatgata aagagcaaga tatcgcagat 4201 aaaatgaaag aagatgaacc ctggcgaata acagataatg aattggagct gtataagacc 4261 aagacatacc ggcagatcag gttaaatgag ttattaaagg aacattcaag cacagctaac 4321 attattgtga tgagtttacc ggtcgcacga aagggtgccg tttctagtgc tctctacatg 4381 gcatggttag aagcactatc taaggatcta ccaccaatcc tactagttcg tgggaatcat 4441 cagagtgtcc ttaccttcta ttcataatta attaagttta aacccgctga tcagcctcga 4501 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 4561 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 4621 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 4681 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 4741 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 4801 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 4861 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 4921 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 4981 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 5041 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 5101 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 5161 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 5221 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 5281 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 5341 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 5401 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 5461 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 5521 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 5581 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 5641 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 5701 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 5761 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 5821 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 5881 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 5941 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 6001 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 6061 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 6121 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 6181 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 6241 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 6301 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 6361 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 6421 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 6481 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 6541 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat 6601 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 6661 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc 6721 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 6781 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 6841 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 6901 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 6961 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 7021 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 7081 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 7141 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 7201 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 7261 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 7321 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 7381 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 7441 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 7501 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 7561 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 7621 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 7681 agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 7741 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 7801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 7861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 7921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 7981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 8041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 8101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 8161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 8221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 8281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 8341 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 8401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 8461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 8521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 8581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 8641 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 8701 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 8761 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 8821 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 8881 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc 8941 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt 9001 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg 9061 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg 9121 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt 9181 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 9241 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 9301 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 9361 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 9421 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 9481 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 9541 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca 9601 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg 9661 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc 9721 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata 9781 gggagaccca agctg //