LOCUS       Exported                1108 bp ds-DNA     linear   SYN 15-JUN-2021
DEFINITION  epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor 
            effector domain. Binds to CIB1 upon blue light stimulation. Synapsin
            promoter for neuronal expression..
ACCESSION   .
VERSION     .
KEYWORDS    LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    21..609
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             127..138
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     536..556
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(967..1020)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gagcagcgct cggtatcgat gcggggaggc ggcccaaagg gagatccgac tcgtctgagg
       61 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
      121 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
      181 aacataggcg agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc
      241 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
      301 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
      361 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
      421 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
      481 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
      541 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
      601 aggttgatta tcgataagct tgatatcgaa ttcttacaca tgatcacttc ccaccagctg
      661 cacaccgatg aagtattgca gccctccctt tcgatcccgg acaggttgga gatgaagcag
      721 attccagaac tttttgcctg acttagtgta gtttattaac tgcacagtgg tttccctctg
      781 atcgcggata gcatcgcgaa tcttctgaac ggtggcctgg tctgtctcag gcccttgcag
      841 gaacctggcg tttctcccca tgatttcctc cctggaatat tcggtgagct ccagaaaacc
      901 gtcgctggca aaaatgatgg ggtaatcagg cagtcttgga tctgttatta cgaaggactt
      961 ctcgattggg ccaggattct cctcgacgtc accgcatgtt agcagacttc ctctgccctc
     1021 tccactgccc catgggggca gcatagaggc atagccatgt tcagcttctc tctcgcgcct
     1081 ctccaggtaa tcggcagcct ccagcagc
//
