LOCUS       Exported                 773 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal 
            alpha-importin nuclear localization sequence. Synapsin promoter for 
            neuronal expression..
ACCESSION   .
VERSION     .
KEYWORDS    LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(29..82)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(104..253)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(278..298)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cggtgaacag ctcctcgccc ttgctcactg ggccaggatt ctcctcgacg tcaccgcatg
       61 ttagcagact tcctctgccc tctccactgc ctctagagtt aatcagcatg tccaggtcga
      121 aatcatcaag ggcgtcactg ccgagcatgt cgaggtcaaa gtcatcaagg gcatccgaac
      181 caagcatgtc aaggtcaaaa tcatcgaggg cgtcacttcc cagcatatcc agatcaaaat
      241 cgtccaatgc gtcagcccgt ccggaaccgc tggcctccac ctttctcttc ttcttggggc
      301 tagccggcgc tgctccgatc ataatctgcg cttcgcgtgt tcttgagatg gctttcgcca
      361 gcaattctcg tgcggtgtca atatcgacga tgggtttcgc gtaattcgtc cccaattcga
      421 cccctgaggc tttaaggacg gtcaggggag catcccacgg gtggtgaatc cactcggtgg
      481 gcagacgggc cagctctggg agccactgcc tgatgtattc tccctccggg tcgtactttg
      541 ctccttgcag tgcagggtta tccaatcggt caagttcatg cccgtcaggg atggaacccg
      601 aaatgtactg ccacccaaga atatcgcact ccaagtccgc atccagaagc gtgtcccaaa
      661 agtatttcat cccccatttc cagggcaaca agaggaactt aacggcaaag ctactaacga
      721 tgactcgaat acggttgtgc atccaacccg ttgcccacag ctctctcata ccc
//
