LOCUS       Exported                1125 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  AAV TALE synthesis backbone with NI half repeat; fused to nuclear 
            localization sequence linker and VP64 transcriptional activator 
            effector domain. Synapsin promoter for neuronal expression..
ACCESSION   .
VERSION     .
KEYWORDS    pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             432..458
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca
       61 aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc
      121 actgccagct tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg
      181 ccgcctcagc actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc
      241 cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg
      301 cgagataggg gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc
      361 tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt cgagaaggat
      421 ccgccaccat gtacccatac gatgttccag attacgctgt agatttgaga actttgggat
      481 attcacagca gcagcaggaa aagatcaagc ccaaagtgag gtcgacagtc gcgcagcatc
      541 acgaagcgct ggtgggtcat gggtttacac atgcccacat cgtagccttg tcgcagcacc
      601 ctgcagccct tggcacggtc gccgtcaagt accaggacat gattgcggcg ttgccggaag
      661 ccacacatga ggcgatcgtc ggtgtgggga aacagtggag cggagcccga gcgcttgagg
      721 ccctgttgac ggtcgcggga gagctgagag ggcctcccct tcagctggac acgggccagt
      781 tgctgaagat cgcgaagcgg ggaggagtca cggcggtcga ggcggtgcac gcgtggcgca
      841 atgcgctcac gggagcaccc ctcaacctga cagagacccg tacgggtctc gactcacgcc
      901 tgagcaggta gtggctattg catccaatat cgggggcaga cccgcactgg agtcaatcgt
      961 ggcccagctt tcgaggccgg accccgcgct ggccgcactc actaatgatc atcttgtagc
     1021 gctggcctgc ctcggcggac gacccgcctt ggatgcggtg aagaaggggc tcccgcacgc
     1081 gcctgcattg attaagcgga ccaacagaag gattcccgag aggac
//
