LOCUS       Exported                 722 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, 
            mutated endogenous NLS, aa318-334 deletion. This particular TALE 
            targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian 
            exp..
ACCESSION   .
VERSION     .
KEYWORDS    LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 cgaagcgggg aggagtcacg gcggtcgagg cggtgcacgc gtggcgcaat gcgctcacgg
       61 gagcacccct caacctgacc ccagagcagg tcgtggcaat tgcgagccat aacgggggaa
      121 agcaggcact cgaaaccgtc cagaggttgc tgcctgtgct gtgccaagcg cacggactta
      181 cgccagagca ggtcgtggca attgcgagca acatcggggg aaagcaggca ctcgaaaccg
      241 tccagaggtt gctgcctgtg ctgtgccaag cgcacggact aaccccagag caggtcgtgg
      301 caattgcgag caacatcggg ggaaagcagg cactcgaaac cgtccagagg ttgctgcctg
      361 tgctgtgcca agcgcacggg ttgaccccag agcaggtcgt ggcaattgcg agcaacggag
      421 ggggaaagca ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc caagcgcacg
      481 gcttaacccc agagcaggtc gtggcaattg cgagccataa cgggggaaag caggcactcg
      541 aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca cggcctgacc ccagagcagg
      601 tcgtggcaat tgcgagcaac atcgggggaa agcaggcact cgaaaccgtc cagaggttgc
      661 tgcctgtgct gtgccaagcg cacggactga caccagagca ggtcgtggca attgcgagca
      721 ac
//
