LOCUS       Exported                 995 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, 
            mutated endogenous NLS, aa318-334 deletion. This particular TALE 
            targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian 
            exp..
ACCESSION   .
VERSION     .
KEYWORDS    LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, 
            Platt RJ, Scott DA, Church GM, Zhang F
  TITLE     Optical control of mammalian endogenous transcription and epigenetic
            states.
  JOURNAL   Nature. 2013 Jul 23. doi: 10.1038/nature12466.
  PUBMED    23877069
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tttcttccat ttcaggtgtc gtgaggatcc gccaccatgt acccatacga tgttccagat
       61 tacgcttcgc ggacccggct cccttcccca cccgcaccca gcccagcgtt ttcggccgac
      121 tcgttctcag acctgcttag gcagttcgac ccctcactgt ttaacacatc gttgttcgac
      181 tcccttcctc cgtttggggc gcaccatacg gaggcggcca ccggggagtg ggatgaggtg
      241 cagtcgggat tgagagctgc ggatgcacca cccccaacca tgcgggtggc cgtcaccgct
      301 gcccgaccgc cgagggcgaa gcccgcacca aggcggaggg cagcgcaacc gtccgacgca
      361 agccccgcag cgcaagtaga tttgagaact ttgggatatt cacagcagca gcaggaaaag
      421 atcaagccca aagtgaggtc gacagtcgcg cagcatcacg aagcgctggt gggtcatggg
      481 tttacacatg cccacatcgt agccttgtcg cagcaccctg cagcccttgg cacggtcgcc
      541 gtcaagtacc aggacatgat tgcggcgttg ccggaagcca cacatgaggc gatcgtcggt
      601 gtggggaaac agtggagcgg agcccgagcg cttgaggccc tgttgacggt cgcgggagag
      661 ctgagagggc ctccccttca gctggacacg ggccagttgc tgaagatcgc gaagcgggga
      721 ggagtcacgg cggtcgaggc ggtgcacgcg tggcgcaatg cgctcacggg agcacccctc
      781 aacctgaccc cagagcaggt cgtggcaatt gcgagccata acgggggaaa gcaggcactc
      841 gaaaccgtcc agaggttgct gcctgtgctg tgccaagcgc acggacttac gccagagcag
      901 gtcgtggcaa ttgcgagcaa catcggggga aagcaggcac tcgaaaccgt ccagaggttg
      961 ctgcctgtgc tgtgccaagc gcacggacta acccc
//
