LOCUS Exported 881 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION to drive the sgRNA expression under a U6 promoter. ACCESSION . VERSION . KEYWORDS sgRNA with U6 promoter SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 881) AUTHORS Chen C, Fenk LA, de Bono M TITLE Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination. JOURNAL Nucleic Acids Res. 2013 Sep 5. PUBMED 24013562 REFERENCE 2 (bases 1 to 881) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..881 /organism="synthetic DNA construct" /mol_type="other DNA" misc_RNA 335..410 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" primer_bind complement(739..756) /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cacgtgtctt gtccaggcgc gccacccaaa tgtttttata atttggcaaa ttaccaaaaa 61 tgtgactgga agcattaaca aaattttgaa caatattcga ggtttcaaaa tcatgatatt 121 ttagatattt tggactgttt tgagtcaaat tgttaatgac agcattttaa gagctcccaa 181 cacatagtgt ttccaatgtt atacccaatc aataatagca agtcaataaa ctacctctac 241 actatttctg gtaataggcg aaacctctac actgtcagtc actttgtaag gtgtgcctat 301 gtatttccat aatatttcat acaaattgaa gcttgtttta gagctagaaa tagcaagtta 361 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgac 421 taatttctgg tattattgtt tagaattgta agaaaatttt acattccaga aaatgtatcc 481 aaaaaactta cgcaaactcg gagcgtatag ttgcatgaag tgctggatcc gaaatacgaa 541 cgacaaattg ttgatacttt gctttcaaac tctcgtaata aggcatcaca aagttctcaa 601 ttttattaat taatggagca caagactggc ctcatgggcc ttccgctcac tgcccgcttt 661 ccagtcggga aacctgtcgt gccagctgca ttaacatggt catagctgtt tccttgcgta 721 ttgggcgctc tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc 781 tggggtgcct aatgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 841 ctggcgtttt tccataggct ccgcccccct gacgagcatc a //