LOCUS Exported 4085 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pIS1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4085) TITLE More Bartel Lab Plasmids REFERENCE 2 (bases 1 to 4085) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4085 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 964..1085 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(975..994) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1029..1048 /label=SV40pA-R /note="SV40 polyA, reverse primer" promoter 1218..1322 /gene="bla" /label=AmpR promoter CDS 1323..2183 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1541..1560) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2354..2942 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2843..2862 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 3017..3768 /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" intron 3838..3970 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 3953..3972 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 4015..4033 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 4043..893 /codon_start=1 /product="luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)" /label=Rluc /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" ORIGIN 1 taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta 61 attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg 121 cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta 181 taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac 241 ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca 301 tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag 361 ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg 421 aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg 481 ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag 541 aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc 601 caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac 661 aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta 721 ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta 781 atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa 841 tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg 901 agctctatac cggtctcgat atcactacta gtgttctaga gcggccgctt cgagcagaca 961 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct 1021 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac 1081 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg 1141 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccaggtggca 1201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 1261 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 1321 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 1381 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 1441 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 1501 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 1561 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 1621 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 1681 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 1741 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 1801 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 1861 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 1921 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 1981 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 2041 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 2101 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 2161 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 2221 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 2281 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 2341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 2401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 2461 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 2521 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 2581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 2641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 2701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 2761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 2821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 2881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 2941 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 3001 tggctcgaca gatctaaatg agtcttcgga cctcgcgggg gccgcttaag cggtggttag 3061 ggtttgtctg acgcgggggg agggggaagg aacgaaacac tctcattcgg aggcggctcg 3121 gggtttggtc ttggtggcca cgggcacgca gaagagcgcc gcgatcctct taagcacccc 3181 cccgccctcc gtggaggcgg gggtttggtc ggcgggtggt aactggcggg ccgctgactc 3241 gggcgggtcg cgcgccccag agtgtgacct tttcggtctg ctcgcagacc cccgggcggc 3301 gccgccgcgg cggcgacggg ctcgctgggt cctaggctcc atggggaccg tatacgtgga 3361 caggctctgg agcatccgca cgactgcggt gatattaccg gagaccttct gcgggacgag 3421 ccgggtcacg cggctgacgc ggagcgtccg ttgggcgaca aacaccagga cggggcacag 3481 gtacactatc ttgtcacccg gaggcgcgag ggactgcagg agcttcaggg agtggcgcag 3541 ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata 3601 tttgcatgtc tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa 3661 ttcgaacacg cagatgcagt cggggcggcg cggtcccagg tccacttcgc atattaaggt 3721 gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaaa gcttgattct 3781 tctgacacaa cagtctcgaa cttaagctgc agaagttggt cgtgaggcac tgggcaggta 3841 agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag 3901 agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt 3961 ctctccacag gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac 4021 gactcactat aggctagcca ccatgacttc gaaagtttat gatccagaac aaaggaaacg 4081 gatga //