LOCUS Exported 4272 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION gene deletion in S. pombe. ACCESSION . VERSION . KEYWORDS pFA6a-ura4MX6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4272) AUTHORS Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E TITLE New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe. JOURNAL Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100. PUBMED 24215641 REFERENCE 2 (bases 1 to 4272) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4272 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(509..1303) /codon_start=1 /gene="Schizosaccharomyces pombe ura4" /product="orotidine 5'-phosphate decarboxylase, required for uracil biosynthesis" /label=S. pombe ura4 /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH" primer_bind complement(1909..1928) /label=T7 /note="T7 promoter, forward primer" promoter complement(1910..1928) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2015..2032) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2186..2774) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2266..2285) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2945..3805) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3568..3587 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3806..3910) /gene="bla" /label=AmpR promoter primer_bind 3978..3996 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(4034..4056) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 4156..4175 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 4256..2 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 4256..1 /label=SP6 /note="SP6 promoter, forward primer" ORIGIN 1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta 61 aggcgcgcca gatctcttgt gatattgacg aaactttttg acatctaatt tattctgttc 121 caacaccaat gtttataacc aagttttatc ttgtttgtct acatggtatt ttacattcat 181 ctacatacat ctttcattgg ctttgtacat agttatcatt acaagtctaa aaaaattcac 241 tcttttctta ttcaatgtca atccaagaga aaagattgtg gtaatgttgt aggagcatgt 301 ttaataaatt actatagcaa attacttttt attcccaagg tgtttatcta taatagttaa 361 tattttagtc gctacataaa attttaccaa agagtacttg tatactaatt ctaaatgcct 421 tctgacataa aacgcctagg aaaacaaacg caaacaaggc atcgactttt tcaataacca 481 accaaaaaaa ttttacatta gtcttttttt aatgctgaga aagtctttgc tgatatgcct 541 tccaaccagc ttctctatat ctcttggctt cgacaacagg attacgacca gctccataga 601 ctccacgacc aacaatgatg atatcgctac cgcagtttac aatcacttct tcaggagtac 661 gatattgctg tcccagcccg tctcctttaa catccaagcc gataccaggg gacatagtta 721 tgtagtcgct ttgaaggtta ggaaatcgac gaccagctat aaagccaaag caaaaatcgg 781 tatgcttctc aaaccattct aaggttttct ctgtgtagga accagtagcc aaagagcctt 841 tggaagacat ttcagccaaa agcaagagac cacgtcccaa aggtaaacca acttctttga 901 ggccttgtat aataccctcg cctggcactg tatggcaatt tgtgatatga gcccaagaag 961 caattttgta cacaccagat gcatattgta gcttgacggt atttccaatg tctgcgaatt 1021 tgcgatcctc aaagataaga aaacgatgct ttttacctaa ggccaccagt ttttctacca 1081 tatcctggtc gaaatcctcg acaacgtcaa tatgtgtctt gataacacag acatagggtc 1141 caattttatc taccaattct aagatttcgg atttcttcgt caaatcgacc gcgactgaca 1201 agttgctttg cttttcttcc atcaaagcca acaattcctt ggcaatggga tttttcatcc 1261 cctcagctct agctgaatag ctttgaaata ctctagcatc cataactttg cttttaaacc 1321 tttaatttcg atccaagcaa aaaagaggtt cttggtagga caatacggta agaaaacacg 1381 acatgtgcag agatgccgac gaagcatagt taaactggga tggtaaaatc aattaagaat 1441 ttataaagac aaaattgtat aagtctctaa aacatcttaa ttatacctca cagaactatc 1501 taaaatatat tcacaaagtg caaacattat catgaaaaag aaccatttta atttaaagca 1561 agggcattaa ggcttattta cagaatttct tacttttgta aagattataa ggctgattat 1621 ctttttcacc atgccaaaaa ttacacaaga tagaatggat gtttgaaatt aaacgtgagt 1681 atacaaacaa atacactagg taaatcgaaa catttttttc tccattaagt aacaaattcc 1741 tatttagaga aagaatgctg agtagattaa ataatctata caaacttttt taacacaaat 1801 gcatacatat agccagtggg atttgtagct aagctgttta aacgagctcg aattcatcga 1861 tgatatcaga tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag 1921 tcgtattaat ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga 1981 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 2041 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 2101 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 2161 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 2221 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 2281 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 2341 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc 2401 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 2461 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 2521 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 2581 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 2641 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 2701 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 2761 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 2821 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 2881 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 2941 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 3001 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 3061 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 3121 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 3181 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 3241 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 3301 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 3361 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 3421 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 3481 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 3541 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 3601 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 3661 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 3721 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 3781 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 3841 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 3901 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 3961 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 4021 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 4081 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 4141 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat 4201 tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta 4261 ggtgacacta ta //