LOCUS       Exported               11078 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  2nd gen bicistronic lentiviral vector allows for simultaneous 
            expression of a transgene and EGFP marker to facilitate tracking of 
            transduced cells..
ACCESSION   .
VERSION     .
KEYWORDS    pWPI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11078)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 11078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3345..3462
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3529..4080
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3673..3690)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3900..3919
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4083..4799
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4083..4799
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4128..4149)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4389..4408)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4736..4757
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4878..4894)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4879..4895)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    4908..5496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4961..4981)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5379..5390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5640..5673
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     LTR             5693..5873
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5898..5916)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5899..5916)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6206..6225)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6439..6458)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6558..6580
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6618..6636)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6704..6808
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6809..7669
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7027..7046)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7840..8428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8329..8348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8582..8599
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8669..8689)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8674..9003
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8854..8989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8916..8935
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          10137..10202
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10332..10352
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10777..10911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10814..10833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10868..10887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg
     3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     3481 ctcgaggttt aaactacggg ctgcaggaat tccgcccccc cccccctaac gttactggcc
     3541 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     3601 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     3661 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     3721 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     3781 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     3841 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     3901 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     3961 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     4021 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     4081 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4141 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4201 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4261 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     4321 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4381 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4441 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4501 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     4561 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4621 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4681 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4741 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4801 ccggactcag atctcgacta gctagtagct agctagctag tcgagctcaa gcttcgaatt
     4861 cgatatcaag cttatcgcga taccgtcgac ctcgagggaa ttccgataat caacctctgg
     4921 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4981 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5041 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5101 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5161 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5221 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5281 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     5341 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5401 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5461 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ggaattcgag ctcggtacct
     5521 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5581 ggactggaag ggctaattca ctcccaacga agacaagatg ggatcaattc accatgggaa
     5641 taacttcgta tagcatacat tatacgaagt tatgctgctt tttgcttgta ctgggtctct
     5701 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5761 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5821 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta
     5881 gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt
     5941 atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct
     6001 ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt
     6061 ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca
     6121 atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     6181 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     6241 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     6301 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     6361 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     6421 cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     6481 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6541 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6601 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6661 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6721 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6781 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6841 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6901 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6961 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7021 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7081 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7141 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7201 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7261 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     7321 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7381 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7441 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7501 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7561 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7621 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7681 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7741 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7801 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7861 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7921 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7981 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8041 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8101 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8161 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8221 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8281 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8341 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8401 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8461 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8521 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8581 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8641 gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8701 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8761 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8821 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8881 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8941 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9001 aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc
     9061 gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc
     9121 gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga
     9181 taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca
     9241 gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat
     9301 tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt
     9361 catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg
     9421 ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc
     9481 ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc
     9541 gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc
     9601 tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa
     9661 tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata
     9721 gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct
     9781 gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg
     9841 cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat
     9901 gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca
     9961 ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat
    10021 tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt
    10081 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
    10141 atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
    10201 agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa
    10261 acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac
    10321 attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat
    10381 tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt
    10441 acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa
    10501 cctttataag taggcataac agttataatc ataacatact gttttttctt actccacaca
    10561 ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa
    10621 tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc
    10681 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    10741 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    10801 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10861 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10921 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    10981 tacctagtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    11041 aaattgtatt tgttaaatat gtactacaaa cttagtag
//
