LOCUS Exported 880 bp ds-DNA linear SYN 24-MAY-2021 DEFINITION gRNA expression empty vector (mammalian cells). ACCESSION . VERSION . KEYWORDS pU6_gRNA_handle_U6t SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 880) AUTHORS Farzadfard F, Perli SD, Lu TK TITLE Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. JOURNAL ACS Synth Biol. 2013 Sep 11. PUBMED 23977949 REFERENCE 2 (bases 1 to 880) AUTHORS . TITLE Direct Submission JOURNAL Exported May 24, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..880 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(189..293) /gene="bla" /label=AmpR promoter promoter 388..628 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 388..408 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 559..578 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 638..713 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" protein_bind complement(725..749) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(809..832) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" ORIGIN 1 gttcttcggg gcgaaaactc tcaaggatct taccgctgnt tgagatccag ttcgatgtaa 61 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 301 ccccgaaaag tgccaccttg tacaaaaaag caggctttaa aggaaccaat tcagtcgact 361 ggatccggta ccaaggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg 421 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag 481 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta 541 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc 601 ttggctttat atatcttgtg gaaaggacgg agctccggtt ttagagctag aaatagcaag 661 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt 721 ctagacccag ctttcttgta caaagttggc attagacgtc gaggaattct accgggtagg 781 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 841 gcgctacaca agtggcctct ggcctcgcac acattccaca //