LOCUS Exported 3938 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Golden Gate entry vector at position 5, small linker insert, final entry vector for the assembly. ACCESSION . VERSION . KEYWORDS pGGEV_5'_Linker SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3938) AUTHORS Kirchmaier S, Lust K, Wittbrodt J TITLE Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. JOURNAL PLoS One. 2013 Oct 7;8(10):e76117. doi: 10.1371/journal.pone.0076117. PUBMED 24116091 REFERENCE 2 (bases 1 to 3938) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3938 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin complement(1..456) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(138..159) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 350..369 /label=F1ori-R /note="F1 origin, reverse primer" promoter 971..989 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 971..988 /label=SP6 /note="SP6 promoter, forward primer" intron 1086..1658 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" polyA_signal 1731..1865 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1755..1774) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1809..1828 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(2017..2034) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2188..2776) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2947..3807) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3570..3589 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3808..3912) /gene="bla" /label=AmpR promoter ORIGIN 1 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctacgca 481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagggc gcctccccca 541 ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc 601 acccaccatt gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc 661 tattgacgtc aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta 721 acttggcaag tacattacta ttggaagtac gccagggtac attggcagta ctcccattga 781 cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg 841 ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa 901 tgggaggtct atataagcaa tgctcgttta gggaaccgcc attctgcctg gggacgtcgg 961 agcaagcttg atttaggtga cactatagag gtctcactgc tgggatccgg cggcggtacc 1021 taagtgagac cctcatcctc ccgggtaact aagtaaggat ctcgaccgat cctgagaact 1081 tcagggtgag tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt 1141 tatatggagg gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc 1201 accatggacc ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt 1261 ctcctcttat tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt 1321 aacgaatttt taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatcac 1381 ttttttttca aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa 1441 ttgttataat taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa 1501 atattcttat tggtagaaac aactacaccc tggtcatcat cctgcctttc tctttatggt 1561 tacaatgata tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct 1621 ctgctaacca tgttcatgcc ttcttctctt tcctacagct cctgggcaac gtgctggttg 1681 ttgtgctgtc tcatcatttt ggcaaagaat taattcctcg acggatcgta gatccagaca 1741 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct 1801 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac 1861 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg 1921 ttttttaatt cgcggccgct ctagaactag tctgcattaa tgaatcggcc aacgcgcggg 1981 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 2041 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 2101 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 2161 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc ggcccccctg acgagcatca 2221 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 2281 gttcccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 2341 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 2401 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 2461 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 2521 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 2581 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 2641 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 2701 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 2761 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 2821 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 2881 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 2941 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 3001 atccatagtt gcctgactgc ccgtcgtgta gataactacg atacgggagg gcttaccatc 3061 tggccccagt gctgcaatga taccgcggga cccacgctca ccggctccag atttatcagc 3121 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 3181 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 3241 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 3301 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgaaa 3361 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 3421 atcactcatg cttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 3481 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 3541 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 3601 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 3661 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 3721 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 3781 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 3841 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 3901 aggggttccg cgcacatttc cccgaaaagt gccacctg //