LOCUS Exported 1049 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS pThy1-Brainbow3.2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1049) AUTHORS Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR TITLE Improved tools for the Brainbow toolbox. JOURNAL Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 2013 May 5. PUBMED 23817127 REFERENCE 2 (bases 1 to 1049) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1049 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 225..246 /label=EGFP-N /note="EGFP, reverse primer" regulatory 288..297 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(306..339) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." polyA_signal 356..477 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(367..386) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 421..440 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS 717..728 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" ORIGIN 1 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg 61 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca 121 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc 181 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc 241 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggccgt 301 acgcgataac ttcgtataaa gtatcctata cgaagttata tgtttaaaca cgccttaaga 361 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 421 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 481 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 541 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctggtgca gcagtctcga 601 cggtatcgat gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga 661 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg 721 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg 781 agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac 841 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata 901 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca 961 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac 1021 aactcctcat aaagagacag caaccagga //