LOCUS       Exported                 850 bp ds-DNA     linear   SYN 15-JUN-2021
DEFINITION  full length coding sequence for zebrafish bozozok gene in gateway 
            middle entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    800 pME-Bozozok
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N
  TITLE     Post-transcriptional mechanisms contribute to Etv2 repression during
            vascular development.
  JOURNAL   Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028.
  PUBMED    24036310
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    75..99
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(685..784)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(801..820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(802..820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(825..841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(825..841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccaatatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
       61 gcttttttat aatgccaact ttgtacaaaa aagcaggctc aaccatggca actcagaagt
      121 tttcaaactt ctccattgat tacatcttag gagacaccag caggcaaaca gcagaatccc
      181 ctggagtgga tcatccagca tcttctcaag atttccaggg gcacttgaga aagctggacc
      241 tgctctacca agaaggatgt agaggtcact gtgatcgtgc agcactgatg gtgaactttc
      301 cttgtccatc atggccaggg atgtactgct gctgcgttcc agtctcctac taccagtcga
      361 cttactatgc agggcaaccg tggccttatg ccgtagccgg ttgtgaaaca gctgagaatc
      421 actaccacac aatcgctcag cgaccgcgcg gtcgaatccg aacggttttt accgacaacc
      481 agacggagca gctcgagcga ctcttcgcgg ttaccgacta cccgaccgtc gagacccggg
      541 cagagttagc ccaaaacacg ggcctcagcg aggagactgt gcgggtgtgg ttcaagaatc
      601 gacgtgcacg cagaaagaga cagacaacct gcgctgacaa acacgctaat cgagctctgg
      661 aggatcatca ggaatcagat tagcacccag ctttcttgta caaagttggc attataagaa
      721 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
      781 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
      841 ggcagctctg
//
