LOCUS Exported 7170 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre. . ACCESSION . VERSION . KEYWORDS pEMS1983 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7170) AUTHORS Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW TITLE New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. JOURNAL Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. PUBMED 30062914 REFERENCE 2 (bases 1 to 7170) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7170 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR intron 2727..2859 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 2842..2861 /label=pMT2-F /note="Synthetic intron, forward primer" polyA_signal 3940..4061 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3951..3970) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4005..4024 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 4231..4360 /label=AAV2 ITR primer_bind complement(4379..4396) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4379..4395) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4388..4410) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4537..4992 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4624..4643) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4834..4855 /label=F1ori-F /note="F1 origin, forward primer" promoter 5018..5122 /gene="bla" /label=AmpR promoter CDS 5123..5983 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5341..5360) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6154..6742 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6643..6662 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6896..6913 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7030..7051 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7066..7096 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7104..7120 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7109..7131 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7128..7144 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7128..7144 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 181 aggaagatcg gaattcccta ggccggccac ctggccacga tgctctagtg taaagcaccc 241 atcagatatc agacgtgaag aacccctcag taacttccta cacctgcaca ttgggcttct 301 ggtcggtggg tccagctgag gatctagcct ctatcaccca aagagagtca gggaaagagg 361 atataccatc atagcacctc tttccacatt acctcaaagc cattttcctc ttcatttgaa 421 aactgagagt tggtgtcctg ggcctgtctc agattgagag gtcctggaaa gccgattgga 481 aaatcctcta agtgggccaa aaatttctgc tcgccaatat ctacttctac atacctccag 541 aaattcatca ttctaaaagc atgtgattgt gcaatagaga tggggaggag agagatgaca 601 attccctagt ccctcctaac cactaatcat catcttacca ggcagaacat cttcctcata 661 gatttcagtg tgacatttat tgagattcta cattatgcct gggaataaca gacatggtcc 721 ttaccctcct aaattttatt atcatagcag acaacacata agctgatccc ttcaatatgg 781 tgggtggtgt aatagaaggt atacactgag ggtcatgaga gcaaggggct aaactgagag 841 ttcagaaaag gcttcccaga gaaaataaga cttgagtgga acattggagg agtaggaatt 901 gacaatataa agatgggtta ctttcacagg aagggccaga ggtgtgcagg acactactag 961 gaaattggag atgaagacaa ggtcaggtcc tgaaggtctt gtatgccagg ctagggagct 1021 tgacctttat cctaagggca atgggagtca ctgaacagtc aagacatgac tgctcagatt 1081 tgccttaggg gcatattgtg tgactgggct taggctcttt tttttttttt tgagacagag 1141 tcttgctctg tcgcccagtc tggagtgcag tggcgcgatc tcagctcact gcaagctctg 1201 cctcctgggt tcacgccatt cttcttcctc agcctcccca gtagctgaga ctacaggcac 1261 ccgccaccaa gcccggctaa ttttttgtat tttttagtag agatggggtt tcaccgtgtt 1321 agccagggtg gtctcaatct cctgaccttg tgatccgccc gcctcggcct cccaaagtgc 1381 tgggattaca ggcatgagcc acagtgtctg gcctgggctt aggctcttcg gagcacccaa 1441 gcaacatagc ctaactggga gaagagaagg agcctgtttc ctggaaattg aggcctaagt 1501 cagagagggc cgaagtcaca gtctgccagg atgttccttg gtcatctgtc ctgtcccttg 1561 caaattccta accagcaggc accaaggcca caccttgccc acaggactta acctggtacc 1621 accagcgaga aaccagccct gctgaaggag ggagagcaca gcctaagcaa gacaagggta 1681 cagactgaaa ggcaatgcct ttggagtgcc cagcccaagc cctgagatcc agcaggaacc 1741 tcctcttaat tgttttgcgc ggggagggag atgatagtta ctcgtccaag ttcactcagt 1801 gatggtgcag ttcgtggcag cgttggaata ctactttggt ttgtatcatt ccaaaccctg 1861 ctgtctccac gaagtcacct tccttggaga agaaggggga aatgaagaag aggaaagata 1921 tgaggaaaga ggagcagaga gaaagagaaa tgagtctttt aaagtggcag aggaaggaga 1981 atggtaaaca gcaggagcga agcggctgag gagaaagaag aggaaagaaa ggcgagacgt 2041 gggaggattg gaacagagac aaaagggagg agagacggac agcgacaagt ggagaaaatc 2101 ggcgaaactt gagtggcaga gaagtctgag cgctgagacc cggcggcccc gtgcgccttc 2161 ccacctggcg ccgatccact ttcctcgggg tagcggccca acccacttcg ctgccagccg 2221 atccctttta cccgtggcta ccgggaccac tctactctcg cccacttggc tctgcctaag 2281 cgtcctagcc ggagcgcggt ctctgccacg tggggagggg cgcggccgag ttgctgaaga 2341 gcgcttctga ttggccagag ggcggggttc ttggcgtctc gccggccaga cccctccctc 2401 aaaggcgggg cctggagatc cacagctgga aagggcggag ccccagcagg acagctggaa 2461 aggggcgggg cctgacgcgc gcggctcgcc gcggcgggct gggggcgccc tggtctgcca 2521 taaagtgaat gggcgccggc tgggggtggc agtacgcggt gaggctcact ccctccgaga 2581 gtccaggagc gcccgagcgg agaggcggcc cgggagcagg ggggcggccc ccactccggc 2641 cgggtgcccg gcccctggcc cctgcctgcc ctctagatcg ccgccgcagc cgggcgcgcc 2701 gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag 2761 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta 2821 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc caggcggccg 2881 ccaccatgtc caacctgctg actgtgcacc aaaacctgcc tgccctccct gtggatgcca 2941 cctctgatga agtcaggaag aacctgatgg acatgttcag ggacaggcag gccttctctg 3001 aacacacctg gaagatgctc ctgtctgtgt gcagatcctg ggctgcctgg tgcaagctga 3061 acaacaggaa atggttccct gctgaacctg aggatgtgag ggactacctc ctgtacctgc 3121 aagccagagg cctggctgtg aagaccatcc aacagcacct gggccagctc aacatgctgc 3181 acaggagatc tggcctgcct cgcccttctg actccaatgc tgtgtccctg gtgatgagga 3241 gaatcagaaa ggagaatgtg gatgctgggg agagagccaa gcaggccctg gcctttgaac 3301 gcactgactt tgaccaagtc agatccctga tggagaactc tgacagatgc caggacatca 3361 ggaacctggc cttcctgggc attgcctaca acaccctgct gcgcattgcc gaaattgcca 3421 gaatcagagt gaaggacatc tcccgcaccg atggtgggag aatgctgatc cacattggca 3481 ggaccaagac cctggtgtcc acagctggtg tggagaaggc cctgtccctg ggggttacca 3541 agctggtgga gagatggatc tctgtgtctg gtgtggctga tgaccccaac aactacctgt 3601 tctgccgggt cagaaagaat ggtgtggctg ccccttctgc cacctcccaa ctgtccaccc 3661 gggccctgga agggatcttt gaggccaccc accgcctgat ctatggtgcc aaggatgact 3721 ctgggcagag atacctggcc tggtctggcc actctgccag agtgggtgct gccagggaca 3781 tggccagggc tggtgtgtcc atccctgaaa tcatgcaggc tggtggctgg accaatgtga 3841 acatagtgat gaactacatc agaaacctgg actctgagac tggggccatg gtgaggctgc 3901 tcgaggatgg ggactgagcg gccgcgatcg cagacatgat aagatacatt gatgagtttg 3961 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta 4021 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc 4081 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct 4141 acaaatgtgg taggtcgaaa ttcccgataa ggatcttcct agagcatggc tacgtagata 4201 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc 4261 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg 4321 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa cctaattcac 4381 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 4441 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 4501 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 4561 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 4621 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 4681 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 4741 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 4801 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 4861 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 4921 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 4981 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 5041 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 5101 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 5161 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 5221 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 5281 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 5341 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 5401 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 5461 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 5521 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 5581 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 5641 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 5701 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 5761 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 5821 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 5881 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 5941 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 6001 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 6061 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 6121 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 6181 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 6241 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 6301 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 6361 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 6421 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 6481 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 6541 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 6601 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 6661 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 6721 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 6781 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 6841 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 6901 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 6961 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 7021 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 7081 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 7141 tgaccatgat tacgccagat ttaattaagg //