LOCUS Exported 6343 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION AAV plasmid with PCP2 (Ple155) promoter driving expression of iCre. Pleiades (Ple) MiniPromoter expression pattern described in Scalabrino et al., 2015 HMG (PMID: 26310623).. ACCESSION . VERSION . KEYWORDS pEMS1985 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6343) AUTHORS Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW TITLE New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. JOURNAL Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. PUBMED 30062914 REFERENCE 2 (bases 1 to 6343) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6343 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR intron 1900..2032 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 2015..2034 /label=pMT2-F /note="Synthetic intron, forward primer" polyA_signal 3113..3234 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3124..3143) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3178..3197 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 3404..3533 /label=AAV2 ITR primer_bind complement(3552..3569) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3552..3568) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3561..3583) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3710..4165 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3797..3816) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4007..4028 /label=F1ori-F /note="F1 origin, forward primer" promoter 4191..4295 /gene="bla" /label=AmpR promoter CDS 4296..5156 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4514..4533) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5327..5915 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5816..5835 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6069..6086 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6203..6224 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6239..6269 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6277..6293 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6282..6304 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6301..6317 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6301..6317 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 181 aggaagatcg gaattcccta ggccggccac gcgtcagcag attgaagaag ccctcctggt 241 ctggggagcc cgcctgggga cagactcgct cagtctggtt ggcccttcag cttgggggcc 301 cctccccaaa cttcctagcc agcttgctca caccctgacc ccggggcctg ccgtccccac 361 ttcctcagct ctcaccatgg tccccgccga tcctctctgc agagctgttc tgaatgagac 421 atgagtctcc ttcccaatcc cggcccccgc cccaggggcc ctggccagca gtgccacttc 481 acgtggtacc gcttcaaggg acaggctccg atgcgtgtcc cgccgtccta gattggggcc 541 tgatgagtgt ggcctgggag ctgggacacg aatcagggaa acatggccca ggagctaccc 601 ccaggtccca gcatctccca tcaatagggg tccacacgga gagccctgcc ctctgccctg 661 gggcctggca ctcagacccc caagcccacc agcccctttc tacagccaca actgggtcag 721 ggggtcctag gagactcact cgttaattag gtgccctaca aactaattag tcttgtcaat 781 catgggctct gagaccttga gctgggggtg gggtgggggc agggccctct cacctcggca 841 caggggcctg agccttcctc cgtcttctcc tcctgatccg gacacttcat tggcatagag 901 ggagagagtg tgaacttggc cctttgtgga acagaggagg ctcgggcaga ggtggtgata 961 gtgcagccca ttcattctga gatgaaactt ccactggttt ccgtaaagac gtcttgggga 1021 gggaagggaa ggggatgggg acctcccagt ggtatcccct gcttgggcac tgagggaaag 1081 ccacagtggc tcggggtaaa aggcagggac atcctctccc cgcctgcctc tgtccccagg 1141 gagtctcgcc tcctgttccc acctggggct agggtgatag aggagaggag atagctcaac 1201 ctggcattta ggtggtgtgg gaacaggaga ccccagactt tcttgttttg gggtctgggg 1261 caggcaacca ggctccaggg acagtgagtt gaaggaaggg tggctgggag accccttgac 1321 ttgctgccaa ggagacagag ctggagctag ggtggcgggt ggtgtctgag gcaggtgcag 1381 agagggaggg agggaagggg cctttgactc caacctcctt tttctttacc gactgcaggt 1441 ggcagctgcc cttccaggag ccagtggggg aacctgggtg gctgggtggg gacacctgca 1501 agtcctccct aagccagcta ccaccctaca ctgttggcct cccttctcca actgtgggga 1561 cgctgctcag gccttttgtg acatcacacc tgagagtccc tggggtccag tcattgctgc 1621 tgggcacagc gaggtccaag ctcaggtcgc cctgccccct acccaccatg ccagatccag 1681 catcgttgtg ggcaaacaat tatctggatg atctttatgg ggcttaagct tgggtgggag 1741 cagatggggc atgagctggg gatttgggga tggggggaat ccacaccccc acgtcctgga 1801 cgtttaaaag gccctctctg gcactgggcc ggggcagagg ccagcagaaa agtgactgga 1861 gtccaggcgc gccgaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga 1921 caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc 1981 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac 2041 tcccaggcgg ccgccaccat gtccaacctg ctgactgtgc accaaaacct gcctgccctc 2101 cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt cagggacagg 2161 caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc 2221 tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt gagggactac 2281 ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca cctgggccag 2341 ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa tgctgtgtcc 2401 ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc caagcaggcc 2461 ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa ctctgacaga 2521 tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct gctgcgcatt 2581 gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg gagaatgctg 2641 atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa ggccctgtcc 2701 ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc tgatgacccc 2761 aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc 2821 caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct gatctatggt 2881 gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc cagagtgggt 2941 gctgccaggg acatggccag ggctggtgtg tccatccctg aaatcatgca ggctggtggc 3001 tggaccaatg tgaacatagt gatgaactac atcagaaacc tggactctga gactggggcc 3061 atggtgaggc tgctcgagga tggggactga gcggccgcga tcgcagacat gataagatac 3121 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa 3181 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac 3241 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc 3301 aagtaaaacc tctacaaatg tggtaggtcg aaattcccga taaggatctt cctagagcat 3361 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg 3421 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc 3481 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat 3541 taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 3601 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc 3661 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt 3721 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 3781 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 3841 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 3901 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 3961 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 4021 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 4081 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 4141 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc 4201 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 4261 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 4321 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 4381 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 4441 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 4501 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 4561 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 4621 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 4681 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 4741 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 4801 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 4861 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 4921 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 4981 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 5041 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 5101 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 5161 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 5221 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 5281 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 5341 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 5401 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 5461 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 5521 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 5581 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 5641 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 5701 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 5761 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 5821 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 5881 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 5941 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 6001 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 6061 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 6121 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 6181 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 6241 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 6301 caggaaacag ctatgaccat gattacgcca gatttaatta agg //