LOCUS       Exported                7494 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  AAV plasmid with FEV (Ple67) promoter driving expression of iCre. 
            Contains WPRE..
ACCESSION   .
VERSION     .
KEYWORDS    pEMS1991
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          2459..2591
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2574..2593
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(3715..3735)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4133..4144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4265..4386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4276..4295)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4330..4349
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4555..4684
                     /label=AAV2 ITR
     primer_bind     complement(4703..4720)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4703..4719)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4712..4734)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4861..5316
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4948..4967)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5158..5179
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5342..5446
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5447..6307
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5665..5684)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6478..7066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6967..6986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7220..7237
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7354..7375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7390..7420
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7428..7444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7433..7455
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7452..7468
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7452..7468
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccac gcgtggcgcg ccggagaaga tcaagactca
      241 ggtgggtgga gatgactgcc ccccatgcac acatttcctg gtgctccacc ccctcagcaa
      301 atgacttctg ggtaagactg agggataaag cctatactgt tgatccttgc ccagctcaca
      361 gctgggatat ttgggtagac ctcactagga ccctctcctt tacaccttag cctcccactg
      421 agagcattaa gtgggcgaat tttgatgcct cccagctttc ccagctctta cctcctgccc
      481 ctggctattt tctgttctta cctcctgctc cttgttttct gtccatagtg ggtgggggaa
      541 gcagaaagag gaatgagaag gagagggatt gggatcttga ccccaacttc aaggccctca
      601 ccttctgtgg agccctgagt ttggtccaca gggaacattc caacctctag tcattgctcc
      661 caccagattc caggtccaca tcctgaaggc ttcatccctg tcccactagg atcctcccag
      721 gccctgggat ccctcttttt ccctccacac tctgcctccc actcttctcc ctggctcctt
      781 tgcaccctcc acgagcaaag atgaacactc cagaagttgc agcaaatgag ggtgaacatg
      841 ggccctgtag aaccccagaa gtaggagttt ccagccatcc tcaatcaggc atcccaggac
      901 caggcactta gcaggagacc tgcccattcc caggcctcct ccacccctgg gctcaagcag
      961 tgagagagga ctttgagaat gccagttttg acagtacccg ttccatactg caaggctgca
     1021 gaaggtgggc ttctgggctg gagcctcatc ttcagccagc atcctcctta gcctgcctct
     1081 ccagagccac gaagccccat aggtacccag agagactcca ggccacttgg gaaccacatc
     1141 aacatctttg catgagttag aaataggaat ccttttcttg gagtggattc cagccttgta
     1201 cagtgcacaa ccctgtctta acccctccac ctctccagcc tcgttcaggg atagccagaa
     1261 tgggagtagt ggtgagggtg ggtggcagac tgggaggaca ggggatgggg aaggatggga
     1321 gcagaatagt tgtacaggga agtagcaggg ggctcaggaa cccatcaggg aaagccagga
     1381 cagattctca acatctttag aggccttcag cagtccaagc cactactaat ttatggggac
     1441 tgaatgaatg agaaaaggag gggagggaga aggatgagag ggagagagaa ggctggaggg
     1501 gagatgagag tcggagagaa aagcctagga ggtaaagaga gacctagaag ggggcgggga
     1561 ggacagaaga gggggccacc catcataagt tgccaggaga gatggcccag caccaggcct
     1621 gaggcttggg ggccccagaa aggcaggaaa cgggccaaag caagaaggag acggggaagg
     1681 ggttcgggtc tctagggaag agcagagagt gggagaagag gaggagggag cggggatgcg
     1741 ggcagagata aagggagcaa cggctgcagg gccgggcgcg cagcgacagg cgagagggag
     1801 ggaagcggaa atttaaaagt gaagatgcag ataacgcagc ctggagacgg gaacccgggt
     1861 ggggatagag ggagggaggg ggaggagagt gagcggcgag tggagcggga gccgactgga
     1921 agaagggctc tagggagggg gctgtggctg ctggggtccg aggtggggcc gggtacacca
     1981 gccccatcac tgtttgcaga gagtcaggga ggcggaaaag acacgcgctc taggctccca
     2041 tcagggcaca tggcccggac ccatcccccg cgcgtctccc cggctgcggg gcgcgggggg
     2101 ctgccgggtg cgcttggctg tggcgcggcg cgttggagac tttattgcga tgggacgata
     2161 agaggggcgg gggcggggtc ctgggggccg aggcggcagc gctttaatta aaacggaaat
     2221 tgcggccccg ggccgcgcgg gggccggagg gttccaagcg gccccttagc tggaagcgtt
     2281 tctccaggac ccccccgcaa cccccgccac gcccgggctg ccccctcccg ccaggccctg
     2341 ccggacccgg cgccgtcttc tcctccttgt cacccgcggt cgcttcgggc ggggatcggt
     2401 gccaccgagc gcaaagcctg cctcggcgcg ccgaagttgg tcgtgaggca ctgggcaggt
     2461 aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca
     2521 gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt
     2581 tctctccaca ggtgtccact cccaggcggc cgccaccatg tccaacctgc tgactgtgca
     2641 ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga agaacctgat
     2701 ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt
     2761 gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc
     2821 tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg tgaagaccat
     2881 ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc ctcgcccttc
     2941 tgactccaat gctgtgtccc tggtgatgag gagaatcaga aaggagaatg tggatgctgg
     3001 ggagagagcc aagcaggccc tggcctttga acgcactgac tttgaccaag tcagatccct
     3061 gatggagaac tctgacagat gccaggacat caggaacctg gccttcctgg gcattgccta
     3121 caacaccctg ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac
     3181 cgatggtggg agaatgctga tccacattgg caggaccaag accctggtgt ccacagctgg
     3241 tgtggagaag gccctgtccc tgggggttac caagctggtg gagagatgga tctctgtgtc
     3301 tggtgtggct gatgacccca acaactacct gttctgccgg gtcagaaaga atggtgtggc
     3361 tgccccttct gccacctccc aactgtccac ccgggccctg gaagggatct ttgaggccac
     3421 ccaccgcctg atctatggtg ccaaggatga ctctgggcag agatacctgg cctggtctgg
     3481 ccactctgcc agagtgggtg ctgccaggga catggccagg gctggtgtgt ccatccctga
     3541 aatcatgcag gctggtggct ggaccaatgt gaacatagtg atgaactaca tcagaaacct
     3601 ggactctgag actggggcca tggtgaggct gctcgaggat ggggactgag cggccgcgat
     3661 cgctcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     3721 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     3781 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     3841 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     3901 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     3961 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4021 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     4081 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     4141 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     4201 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gatcgcagac
     4261 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     4321 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4381 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     4441 gttttttaaa gcaagtaaaa cctctacaaa tgtggtagtc gaaattcccg ataaggatct
     4501 tcctagagca tggctacgta gataagtagc atggcgggtt aatcattaac tacaaggaac
     4561 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     4621 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     4681 gcagccttaa ttaacctaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4741 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4801 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     4861 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4921 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4981 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5041 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5101 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5161 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5221 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5281 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     5341 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5401 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5461 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5521 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5581 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5641 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5701 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5761 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5821 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5881 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5941 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6001 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6061 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6121 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6181 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6241 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6301 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6361 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6421 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6481 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6541 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6601 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6661 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6721 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6781 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6841 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6901 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6961 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7021 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7081 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7141 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7201 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7261 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7321 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     7381 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7441 acaatttcac acaggaaaca gctatgacca tgattacgcc agatttaatt aagg
//
