LOCUS Exported 7281 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION AAV plasmid with OLIG1 (Ple304) promoter driving expression of iCre. . ACCESSION . VERSION . KEYWORDS pEMS1993 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7281) AUTHORS Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW TITLE New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. JOURNAL Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. PUBMED 30062914 REFERENCE 2 (bases 1 to 7281) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7281 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR intron 2838..2970 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 2953..2972 /label=pMT2-F /note="Synthetic intron, forward primer" polyA_signal 4051..4172 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4062..4081) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4116..4135 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 4342..4471 /label=AAV2 ITR primer_bind complement(4490..4507) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4490..4506) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4499..4521) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4648..5103 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4735..4754) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4945..4966 /label=F1ori-F /note="F1 origin, forward primer" promoter 5129..5233 /gene="bla" /label=AmpR promoter CDS 5234..6094 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5452..5471) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6265..6853 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6754..6773 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7007..7024 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7141..7162 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7177..7207 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7215..7231 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7220..7242 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7239..7255 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7239..7255 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 181 aggaagatcg gaattcccta ggccggccac tgagttaata tttccaggtc agagcgcctc 241 cgagagaaaa aggcggtggc cttggagctc tggtctcctt aactgcaccc tgggaagtct 301 tcacacggcc tttcttcccc tggtggtcta atctggagtc agatccagcc gtcagctctt 361 acagacgggg aaggaccggg agccaacggg aaaacaacag gtgagcttgt gaacaagtcc 421 ccttctgtgt ctcgggcagg gtgtctgccc gggcaaccgt ggggctgcac tgacttccag 481 aaccttctgc ctctgcaggt gttttaggaa cttggcctca gcgcttcagt gacccatatt 541 ttattacctt tcaggtttct ataattcatc attcactcct ttctgaaaac ctagaatgaa 601 aaagtttagc agctcagcgc cgcggcctct ctccattctc tccccaaaca catcggcctg 661 tttgttctct ttcatccccg ctcttcctgt tgcattctgc cggttcaatg gggaaggagt 721 gaaaccggat ggctggaccc agtgcgggga aatgacttca gagccacctt tgtttccctc 781 attcctttga gcgcacttgg ccctgcgtct ctatgaaagg gcttgtttga accattcgga 841 acatccagca agccagagat gctgcggcca gaaccgctcg catcttttca ggctgcctga 901 gactctgggt taaactgcca aatttttgca acaaaactaa ccatgacatt ggacattgtg 961 ttataaatta gccacagcct tccaagcaaa ttgtctcttt ttattgtatc agtgtaagcc 1021 tcggaaacat ttggcttcag ctactacctt cagggaggct cagaggatat ttttgttctg 1081 ccccaaacca actcttcaga caccaccttc cttgtcctca cgcttgacta gcagacaatg 1141 ggttcttcag aagccacagc atttcagctg ttttggcccc agaggccaca agctgactgc 1201 atgtcattct ccaccagcag agcgtcacct cggggtagct ccaaacagta tcaaccggtt 1261 tgtggtgagt ggataaacac caggctgggt gaatgaagtc acaggctgag tcatcctctg 1321 cacatggggg ctgaatgggg ctcagtcagg cccagaggag cctccccggc aaggtgctgg 1381 gggccaggct ctccctgcca gtgaggctgg ggtccgtccc caatagtcat tcctttggcc 1441 aacagaaatg ataaggcaga caaatggaag atcaatgaag caacatcgtg gcaataatgc 1501 aattggtcct gccgtagtca tgaacatgta aataacaaga cagcaataag aagaccccag 1561 actctgccta gaaacaaaat attcagcctg ttgttgttgt ttcttattgc aggattttga 1621 gtggaaaagt agttccactt gtcaatcagc tacgttgcag gggctaatag attctggtgt 1681 ttagggaggt caacacggaa tttaatttaa catctcacaa tacacccaca tgcccagaaa 1741 atgtggacag cttgataggg ttcactgagc caaagaagca agacccaaac agtgcattaa 1801 tgtatagggg aagaatagtt gcaatcagcc ccttgtgaat tggacagagg gcttggagat 1861 gaagcaatta tcagccatcg gggattaata gtaaagtgtg aataccaagc tgtatatgtt 1921 aggggcagaa tatgttgcct atagaaaaca acaaaggacg tttcttacta ttaagtgaga 1981 ttaaacgtaa tggcctgatt taatcacaaa ggcagaatta aattcagatg gcaacttcaa 2041 agtggacaca ggaacacagg ccactgctta catttacata tagctggcat agcttggcct 2101 gcaacacaat aggataaagt ttctttggcc acttaaacaa ttttctattc ttcacctttt 2161 tctttttttc tttttttttt cttttgtggg tgtgtgtgtg tgtgtgtgtg tgtgtgaaca 2221 ctcactgtat ttattcccca attgcattta gttagtaaga aggagctaag tgttatattt 2281 attcatggag gctataactg ttaattcttc caaattgtga aaaaaaagcc tcatagggtc 2341 aataagaaca gagtagttat aatcagattg tcaccaaaaa agaaaaagaa gaaaaaggtt 2401 gtttttgagt agacagttgc tggaggaagg cacgtgagtt gcagcctgac cagtgcgacc 2461 agtcctcctt caacagcccc gcttgccttg aaaaggcgcc cttactccgc tcagggggcc 2521 tgtcccgcgc ggctggcaca ggcgctcaca ataacctcct aaagcggtgc cagccgcacc 2581 tccgcgcggc cccggcacaa gcagccaatg aacacgcggc tgcgcccggc ctcgcgcctc 2641 cattggctgc gccccgccac ccgctgcccc gcaggttccc aagccgggtt taaaggggta 2701 gggcgcgggc cagggcccca ccatcgtttc cccgcgcgca ggtccgcggg gaggggcggc 2761 ctgccgaccg gcccacccca gggcgttcct gaagggcgtc ctcggcgcgc cgaagttggt 2821 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga 2881 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta 2941 ctgacatcca ctttgccttt ctctccacag gtgtccactc ccaggcggcc gccaccatgt 3001 ccaacctgct gactgtgcac caaaacctgc ctgccctccc tgtggatgcc acctctgatg 3061 aagtcaggaa gaacctgatg gacatgttca gggacaggca ggccttctct gaacacacct 3121 ggaagatgct cctgtctgtg tgcagatcct gggctgcctg gtgcaagctg aacaacagga 3181 aatggttccc tgctgaacct gaggatgtga gggactacct cctgtacctg caagccagag 3241 gcctggctgt gaagaccatc caacagcacc tgggccagct caacatgctg cacaggagat 3301 ctggcctgcc tcgcccttct gactccaatg ctgtgtccct ggtgatgagg agaatcagaa 3361 aggagaatgt ggatgctggg gagagagcca agcaggccct ggcctttgaa cgcactgact 3421 ttgaccaagt cagatccctg atggagaact ctgacagatg ccaggacatc aggaacctgg 3481 ccttcctggg cattgcctac aacaccctgc tgcgcattgc cgaaattgcc agaatcagag 3541 tgaaggacat ctcccgcacc gatggtggga gaatgctgat ccacattggc aggaccaaga 3601 ccctggtgtc cacagctggt gtggagaagg ccctgtccct gggggttacc aagctggtgg 3661 agagatggat ctctgtgtct ggtgtggctg atgaccccaa caactacctg ttctgccggg 3721 tcagaaagaa tggtgtggct gccccttctg ccacctccca actgtccacc cgggccctgg 3781 aagggatctt tgaggccacc caccgcctga tctatggtgc caaggatgac tctgggcaga 3841 gatacctggc ctggtctggc cactctgcca gagtgggtgc tgccagggac atggccaggg 3901 ctggtgtgtc catccctgaa atcatgcagg ctggtggctg gaccaatgtg aacatagtga 3961 tgaactacat cagaaacctg gactctgaga ctggggccat ggtgaggctg ctcgaggatg 4021 gggactgagc ggccgcgatc gcagacatga taagatacat tgatgagttt ggacaaacca 4081 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat 4141 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt 4201 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg 4261 gtaggtcgaa attcccgata aggatcttcc tagagcatgg ctacgtagat aagtagcatg 4321 gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc 4381 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc 4441 gggcggcctc agtgagcgag cgagcgcgca gccttaatta acctaattca ctggccgtcg 4501 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 4561 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 4621 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg 4681 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 4741 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 4801 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 4861 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 4921 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 4981 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 5041 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa 5101 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 5161 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 5221 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 5281 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 5341 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 5401 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 5461 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 5521 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 5581 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 5641 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 5701 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 5761 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 5821 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 5881 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 5941 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 6001 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 6061 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 6121 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 6181 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 6241 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 6301 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 6361 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 6421 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 6481 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 6541 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 6601 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 6661 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 6721 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 6781 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 6841 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 6901 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 6961 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 7021 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 7081 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 7141 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 7201 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 7261 ttacgccaga tttaattaag g //