LOCUS       Exported                7711 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  AAV plasmid with UGT8 (Ple267) promoter driving expression of iCre..
ACCESSION   .
VERSION     .
KEYWORDS    pEMS1996
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          3269..3401
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3384..3403
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    4482..4603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4493..4512)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4547..4566
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4772..4901
                     /label=AAV2 ITR
     primer_bind     complement(4920..4937)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4920..4936)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4929..4951)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5078..5533
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5165..5184)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5375..5396
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5559..5663
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5664..6524
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5882..5901)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6695..7283
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7184..7203
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7437..7454
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7571..7592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7607..7637
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7645..7661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7650..7672
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7669..7685
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7669..7685
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccac gcgtggcgcg taaagtattt ctaggaaagt
      241 acaaactatt taaaatcatt agttagtttt gaactaaaat cctttaaaaa tatacaacaa
      301 aaaaggtagt ttccagatgt cctttacact gttactgaga atgtaaaatg tctttcatta
      361 atttggattc tttaaacatt cctctatgat catatttaag tgtgaaattt tactccctat
      421 tgttgtagat aatcttttag tcaggtaatg aatagcacat ggtacagaaa aacatataca
      481 tatacatttt catttcagct tttctgaagc cccattgttt atttcaggct gctttactga
      541 gactccttgc actaaacgcc tctggttgca ttccactcaa gacaaaggca tattttaggg
      601 atgtacctct gacaaagagg tatttatgga ccaggaatca gactttggac aaatgggcat
      661 ttcattataa cacagcctaa ttgttaaagc tcttattgaa aagaataaaa atttcattag
      721 tcatcacagg aactcagcac aaactcctcc tccttttccc cacaatgcac agaaagaatg
      781 gcagctttaa gcttatcatt gaacattttg aaggctttgg tccttccttc cttttgttcc
      841 acttgcacta agaagtattt tagtaacaat tgtgaaataa ctgtggtaat aattttaacg
      901 aaattttata aatgtatctt ctgctcctaa gtgcagcaca ctgggggaaa ggaaaggcca
      961 aaagagggag ggtcatcaac tgcagagttc cacggggttc caggtgattg caggttataa
     1021 agcagcttgg tgcctgtata ggcttagtaa atcaagaagt gatgttaaga taatggaaac
     1081 agcctgcttt gtataaattt gtttactctg ttctcaatag gtaacataat ttgcatcata
     1141 tgctctattt atgacacacc tggattcaga aatgaggtca ttaagttata aacttcatct
     1201 ggttcttttg tgaaaaatag acatcaacta ttagcccact tataaaataa cctgtataaa
     1261 ttgtaccacc tgtctccatg aagcctcttc aagtgttcac aacactgatt tcttgctttt
     1321 atcatttcaa actccaaaag tgtatttcaa ctattcatat gatgattgaa taaacttgtt
     1381 ttacattatg gaacaccctt actgtgtaga gctgtgagaa attcaataat cagtatttaa
     1441 tgtggacaaa aatagataga ttagagaaac aataaacatg gacatatcta cacataatat
     1501 atactccttt tctatccaaa aatctgacaa aaaccttaca caatggtcag tttacaccat
     1561 tttaaggaca aggatgtggt gaaacaattt taaacatttt tatataataa aagaaaaaag
     1621 ttgttttaat tgctaatgat caagttttta ccaagaattt acatatgcat catcaaacag
     1681 tagcttgaaa ctgaagcttg acctttgaga aataaagctt ttaaagtaaa tgtctaaatt
     1741 ctcttttctt tattatgtta catttcattt actttaggtt ttctagtgca atttaaataa
     1801 gaaataaata gagagtatgg taatcaggta caaaggctgt gaaaatattc cctgcatttt
     1861 gcacagggaa tatgttcaaa ttcctaatct gcaaagaaga aaagtgcatt ttcttttttg
     1921 aaatggcatt tgaagatctc tccatccatg aacattcttg agcatatgga ttttacaata
     1981 acagcagtgt atattagttt tttaatttat cattaactga taacatatac agtaaaatat
     2041 aatttccgtt ttcttatcta agggcagaaa atcccaacct atttctgaat ctcactttgg
     2101 aacaaggaca gaatgacctg acatggcatt tttgatttcc aacgtttaaa cgcataacgt
     2161 ttgtgtgcta tggaagcatc ttagcttctg gaatttatgc tcgacacgaa acataaacaa
     2221 taaacaattc agtccacgtt atatagacat atctcatata tatatgagat atatatatat
     2281 aaaatatctt acgcacatgc acacttagac tttctcggtt ttcatgaaac tcacaatcta
     2341 cctcaggcgc tcaaaggcac tcggcctctc aggtctgagg caccacagag aggcttcctt
     2401 gggcacagtt gcttgctggt caagacgcca acttggcaag gttatgctgc tggcaaaggc
     2461 agattcgtca gaataaagtc gccacaggct caaccaggca aatcatgaat ggccctttca
     2521 gcaggagcct gagaggaggg atgttattca gcccagcaac cctatttact ttttttcagg
     2581 atccccaaac tcgcgttttt taaggctttc cgctacagca cagggactac gtgggtggtg
     2641 gcagaaaggg cgcgggacac gcctcgcaaa gagggaagag tgggcggggc cacgtgccgg
     2701 tgtcagagtt cgcaactcga gcgcccagag ggctcgcgaa aagtcccagc ctgcaagcca
     2761 accgcgctca gcggacgact ggccggatcc caacgcgctg ccccttgccc agcctgcgag
     2821 cgcgtggtac gaaggcgcgt ctgcatccat gccccagccc ggggagctgg aggcgctcgc
     2881 agtcagaggc gagtgatgct aggctgagcg cgtggcggcc cgtgtcgtgc cccgctgagc
     2941 caagtgcgga agggcagcgg cgcgctccag ctctgctcgc cgcgcgcagg gcgggggggg
     3001 ctggccgccc gctgggagct gcggacgagc aggcgcgctg aggacccgag ggaggacacg
     3061 gttaaagcat tgctatcaac tgtgaaccca gagagccctc cttagccaac acgctaactc
     3121 cgaagcctcc cttacgcccc cgaaccaccg aaggcggcga cacctgattc agcgcacaaa
     3181 cacaggtccc ttctgtcccg gatacaatta cgcggcagac acacactcaa actcggcgcg
     3241 ccgaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg
     3301 agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     3361 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact cccaggcggc
     3421 cgccaccatg tccaacctgc tgactgtgca ccaaaacctg cctgccctcc ctgtggatgc
     3481 cacctctgat gaagtcagga agaacctgat ggacatgttc agggacaggc aggccttctc
     3541 tgaacacacc tggaagatgc tcctgtctgt gtgcagatcc tgggctgcct ggtgcaagct
     3601 gaacaacagg aaatggttcc ctgctgaacc tgaggatgtg agggactacc tcctgtacct
     3661 gcaagccaga ggcctggctg tgaagaccat ccaacagcac ctgggccagc tcaacatgct
     3721 gcacaggaga tctggcctgc ctcgcccttc tgactccaat gctgtgtccc tggtgatgag
     3781 gagaatcaga aaggagaatg tggatgctgg ggagagagcc aagcaggccc tggcctttga
     3841 acgcactgac tttgaccaag tcagatccct gatggagaac tctgacagat gccaggacat
     3901 caggaacctg gccttcctgg gcattgccta caacaccctg ctgcgcattg ccgaaattgc
     3961 cagaatcaga gtgaaggaca tctcccgcac cgatggtggg agaatgctga tccacattgg
     4021 caggaccaag accctggtgt ccacagctgg tgtggagaag gccctgtccc tgggggttac
     4081 caagctggtg gagagatgga tctctgtgtc tggtgtggct gatgacccca acaactacct
     4141 gttctgccgg gtcagaaaga atggtgtggc tgccccttct gccacctccc aactgtccac
     4201 ccgggccctg gaagggatct ttgaggccac ccaccgcctg atctatggtg ccaaggatga
     4261 ctctgggcag agatacctgg cctggtctgg ccactctgcc agagtgggtg ctgccaggga
     4321 catggccagg gctggtgtgt ccatccctga aatcatgcag gctggtggct ggaccaatgt
     4381 gaacatagtg atgaactaca tcagaaacct ggactctgag actggggcca tggtgaggct
     4441 gctcgaggat ggggactgag cggccgcgat cgcagacatg ataagataca ttgatgagtt
     4501 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     4561 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     4621 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     4681 ctacaaatgt ggtagtcgaa attcccgata aggatcttcc tagagcatgg ctacgtagat
     4741 aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact
     4801 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4861 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gccttaatta acctaattca
     4921 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4981 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5041 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     5101 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5161 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5221 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5281 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5341 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5401 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5461 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5521 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5581 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5641 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5701 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5761 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5821 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5881 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5941 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6001 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6061 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6121 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6181 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6241 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6301 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6361 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6421 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6481 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6541 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6601 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6661 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6721 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6781 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6841 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6901 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6961 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7021 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7081 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7141 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7201 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7261 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7321 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7381 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7441 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7501 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7561 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7621 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7681 atgaccatga ttacgccaga tttaattaag g
//
