LOCUS Exported 5672 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION AAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.. ACCESSION . VERSION . KEYWORDS pEMS2022 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5672) AUTHORS Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW TITLE New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. JOURNAL Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. PUBMED 30062914 REFERENCE 2 (bases 1 to 5672) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5672 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR CDS 388..399 /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" intron 1230..1362 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 1345..1364 /label=pMT2-F /note="Synthetic intron, forward primer" polyA_signal 2443..2564 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2454..2473) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2508..2527 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 2733..2862 /label=AAV2 ITR primer_bind complement(2881..2898) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(2881..2897) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2890..2912) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3039..3494 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3126..3145) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3336..3357 /label=F1ori-F /note="F1 origin, forward primer" promoter 3520..3624 /gene="bla" /label=AmpR promoter CDS 3625..4485 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3843..3862) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4656..5244 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5145..5164 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5398..5415 /label=L4440 /note="L4440 vector, forward primer" protein_bind 5532..5553 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 5568..5598 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5606..5622 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 5611..5633 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 5630..5646 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 5630..5646 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 181 aggaagatcg gaattcccta ggccggcctc tacttgtggg agaggacagg agggagaggt 241 gccgtggtga gactgaccct cgggcccacg ggtggatgat ggcaaaggtc ctgtggcagg 301 tgtgggagaa gcagggctgc ggccagctgc cctgagaggc ttcaaattca ggccagatct 361 gctctgggca agaggaagct gtgggtttgg gagcttcaga gacaggggag caggcctgtc 421 actcaccctc ttttccttaa aggctgtcac ccccagcagt gcaccctgca gcctgcctat 481 cccctgtgca gctccagctc cgtctgtccc cagcaatgca ccccacgccc atgtccccca 541 ctccctatga tgctctcgcg ccttctggag cagcagtggt accagctgga ggctgtggaa 601 aaattgagag gagacaaaag tgggaagagc aggccgtgga gagagtgtct aaacacaggg 661 actatttgga gattgtctta tttttggttt gaaatgggcc cacctgctag tattgtacaa 721 acggctctgc tcgctaggcc aggcctgcag tggctacggg cctatccttc actggtcagc 781 ctggagcagc tcctcggacc tcagtctgct catccgtggg tagaggcgac gcagacccca 841 tcccactggg gactgggcca gacagcctgt gaggcagtca gctggtgcct ggccagaggg 901 tgtctgaaag gcctcttggc tgcagggtgc atctgctttt gggacagctc ttcagagcca 961 tctcagaagg gacagcatcc gcctgtctac ttcaactccc actgatgacg tccatgtgtc 1021 attagtgcca attagaggag ggcagcaggc tgagtgcttg gcctggggcg caagcttgtg 1081 ggagggataa ttggattacc cgctagacta atgtgattac ccgtgctgcc tggacccacc 1141 ccattcagga ccagggcata aatggccagg tgggaccaga gagctcaccc cagccggcgc 1201 gccgaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag 1261 gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac 1321 ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac tcccaggcgg 1381 ccgccaccat gtccaacctg ctgactgtgc accaaaacct gcctgccctc cctgtggatg 1441 ccacctctga tgaagtcagg aagaacctga tggacatgtt cagggacagg caggccttct 1501 ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc 1561 tgaacaacag gaaatggttc cctgctgaac ctgaggatgt gagggactac ctcctgtacc 1621 tgcaagccag aggcctggct gtgaagacca tccaacagca cctgggccag ctcaacatgc 1681 tgcacaggag atctggcctg cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga 1741 ggagaatcag aaaggagaat gtggatgctg gggagagagc caagcaggcc ctggcctttg 1801 aacgcactga ctttgaccaa gtcagatccc tgatggagaa ctctgacaga tgccaggaca 1861 tcaggaacct ggccttcctg ggcattgcct acaacaccct gctgcgcatt gccgaaattg 1921 ccagaatcag agtgaaggac atctcccgca ccgatggtgg gagaatgctg atccacattg 1981 gcaggaccaa gaccctggtg tccacagctg gtgtggagaa ggccctgtcc ctgggggtta 2041 ccaagctggt ggagagatgg atctctgtgt ctggtgtggc tgatgacccc aacaactacc 2101 tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc caactgtcca 2161 cccgggccct ggaagggatc tttgaggcca cccaccgcct gatctatggt gccaaggatg 2221 actctgggca gagatacctg gcctggtctg gccactctgc cagagtgggt gctgccaggg 2281 acatggccag ggctggtgtg tccatccctg aaatcatgca ggctggtggc tggaccaatg 2341 tgaacatagt gatgaactac atcagaaacc tggactctga gactggggcc atggtgaggc 2401 tgctcgagga tggggactga gcggccgcga tcgcagacat gataagatac attgatgagt 2461 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg 2521 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca 2581 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc 2641 tctacaaatg tggtagtcga aattcccgat aaggatcttc ctagagcatg gctacgtaga 2701 taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac 2761 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc 2821 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agccttaatt aacctaattc 2881 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 2941 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 3001 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt 3061 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 3121 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 3181 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 3241 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 3301 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 3361 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 3421 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 3481 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 3541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 3601 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 3661 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 3721 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 3781 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 3841 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 3901 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 3961 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 4021 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 4081 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 4141 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 4201 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 4261 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 4321 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 4381 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 4441 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 4501 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 4561 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 4621 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 4681 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 4741 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 4801 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 4861 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 4921 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 4981 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 5041 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 5101 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 5161 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 5221 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 5281 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 5341 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 5401 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 5461 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 5521 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 5581 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc 5641 tatgaccatg attacgccag atttaattaa gg //