LOCUS Exported 5894 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION AAV plasmid with TNNT1-B (Ple301) promoter driving expression of iCre.. ACCESSION . VERSION . KEYWORDS pEMS2026 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5894) AUTHORS Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW TITLE New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. JOURNAL Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. PUBMED 30062914 REFERENCE 2 (bases 1 to 5894) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5894 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR intron 1452..1584 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 1567..1586 /label=pMT2-F /note="Synthetic intron, forward primer" polyA_signal 2665..2786 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2676..2695) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2730..2749 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 2955..3084 /label=AAV2 ITR primer_bind complement(3103..3120) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3103..3119) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3112..3134) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3261..3716 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3348..3367) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3558..3579 /label=F1ori-F /note="F1 origin, forward primer" promoter 3742..3846 /gene="bla" /label=AmpR promoter CDS 3847..4707 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4065..4084) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4878..5466 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5367..5386 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5620..5637 /label=L4440 /note="L4440 vector, forward primer" protein_bind 5754..5775 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 5790..5820 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5828..5844 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 5833..5855 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 5852..5868 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 5852..5868 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 181 aggaagatcg gaattcccta ggccggccga aaacttagag tgccattatc tggggtatca 241 tgaactgggc ctccgagttt gggtatcaag attttggggg tctcatattc ttgaggtttc 301 agattttagg aggtctcaag acctgggggt ggtcttagga tgtcccggcc catatctgga 361 ctatcaagaa ctgggatttc agaatccaga gctgtgagga cttgctggcc tcagaaacac 421 gggcatcata aatcggggtc tcagaatctg tgggtattgg gatttggggc taggcaccct 481 gtggcctagg gtgtctcaaa gactttgagg tctaagaatg taggggtcag ggtgaggtct 541 acggacagaa ggtgcccttt atgcccctgc cccctacgtg atgtatggga aggtgaaagg 601 ggagacaggt ggggaggaaa ccagccagtg gggtggggcg agtgggagag gggatgcggg 661 tgactaattc tcccagaagc cccccttcct caaatccctt cttaggaaat gtcctcggtt 721 tcaccatcta tgacatcccc ccaaaatagc tcctgggggt ggggcagcta ttgtcttcag 781 gccactgtcc cttctcaaat gcctcttcct aatcccaact ggatcaggtt cccatggact 841 tgtcataaga caaaagagga cagctgtgct gagggggcag ggtctgcagc ctcctggctg 901 tgccaggacc acacctacca aggtctgtcc tcatgcatgc tttaggacag ccggccccct 961 ccctcagacc caagagtcca gacctgagcc ctcctccctc agacgcagga gtacaggccc 1021 ccagcccgct cctccctcag actcaggagt ctagactccc agcctctccc ctccaaggac 1081 ctactgtgtg ccgggcatgg ggacgtcaaa gcacagggag ctataaggga ggtagttgga 1141 gcaccacggg agcctggggt gtggggtggg agcaggggcg gggctcctgg gccatgcaga 1201 tggggttggc atggggttgg ggaggggccc acggaggctt ctggactctg agtgtggagc 1261 caggggcagc agggctgtct ctgaactccc aggcttgtgt ttgagcaaag gaattctctc 1321 cccctccctc tcccctcggg gcgggcccga gcctccagct ataaacttcc cggagcttct 1381 gtgccctcag caaggctcag cctcaagatt cacagcaggc gcgccgaagt tggtcgtgag 1441 gcactgggca ggtaagtatc aaggttacaa gacaggttta aggagaccaa tagaaactgg 1501 gcttgtcgag acagagaaga ctcttgcgtt tctgataggc acctattggt cttactgaca 1561 tccactttgc ctttctctcc acaggtgtcc actcccaggc ggccgccacc atgtccaacc 1621 tgctgactgt gcaccaaaac ctgcctgccc tccctgtgga tgccacctct gatgaagtca 1681 ggaagaacct gatggacatg ttcagggaca ggcaggcctt ctctgaacac acctggaaga 1741 tgctcctgtc tgtgtgcaga tcctgggctg cctggtgcaa gctgaacaac aggaaatggt 1801 tccctgctga acctgaggat gtgagggact acctcctgta cctgcaagcc agaggcctgg 1861 ctgtgaagac catccaacag cacctgggcc agctcaacat gctgcacagg agatctggcc 1921 tgcctcgccc ttctgactcc aatgctgtgt ccctggtgat gaggagaatc agaaaggaga 1981 atgtggatgc tggggagaga gccaagcagg ccctggcctt tgaacgcact gactttgacc 2041 aagtcagatc cctgatggag aactctgaca gatgccagga catcaggaac ctggccttcc 2101 tgggcattgc ctacaacacc ctgctgcgca ttgccgaaat tgccagaatc agagtgaagg 2161 acatctcccg caccgatggt gggagaatgc tgatccacat tggcaggacc aagaccctgg 2221 tgtccacagc tggtgtggag aaggccctgt ccctgggggt taccaagctg gtggagagat 2281 ggatctctgt gtctggtgtg gctgatgacc ccaacaacta cctgttctgc cgggtcagaa 2341 agaatggtgt ggctgcccct tctgccacct cccaactgtc cacccgggcc ctggaaggga 2401 tctttgaggc cacccaccgc ctgatctatg gtgccaagga tgactctggg cagagatacc 2461 tggcctggtc tggccactct gccagagtgg gtgctgccag ggacatggcc agggctggtg 2521 tgtccatccc tgaaatcatg caggctggtg gctggaccaa tgtgaacata gtgatgaact 2581 acatcagaaa cctggactct gagactgggg ccatggtgag gctgctcgag gatggggact 2641 gagcggccgc gatcgcagac atgataagat acattgatga gtttggacaa accacaacta 2701 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa 2761 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg 2821 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtagtc 2881 gaaattcccg ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt 2941 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg 3001 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc 3061 ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat tcactggccg tcgttttaca 3121 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 3181 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 3241 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 3301 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 3361 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 3421 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 3481 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 3541 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 3601 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 3661 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt 3721 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 3781 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 3841 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 3901 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 3961 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 4021 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 4081 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 4141 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 4201 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 4261 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 4321 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 4381 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 4441 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 4501 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 4561 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 4621 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 4681 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 4741 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 4801 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 4861 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 4921 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 4981 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 5041 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 5101 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 5161 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 5221 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 5281 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 5341 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 5401 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 5461 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 5521 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 5581 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 5641 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 5701 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 5761 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 5821 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc 5881 agatttaatt aagg //