LOCUS       Exported                 813 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  Expresses APEX2 in the cytosol of mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3 APEX2-NES
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             28..774
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             781..807
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 atggactaca aggatgacga cgataaggga aagtcttacc caactgtgag tgctgattac
       61 caggacgccg ttgagaaggc gaagaagaag ctcagaggct tcatcgctga gaagagatgc
      121 gctcctctaa tgctccgttt ggcattccac tctgctggaa cctttgacaa gggcacgaag
      181 accggtggac ccttcggaac catcaagcac cctgccgaac tggctcacag cgctaacaac
      241 ggtcttgaca tcgctgttag gcttttggag ccactcaagg cggagttccc tattttgagc
      301 tacgccgatt tctaccagtt ggctggcgtt gttgccgttg aggtcacggg tggacctaag
      361 gttccattcc accctggaag agaggacaag cctgagccac caccagaggg tcgcttgccc
      421 gatcccacta agggttctga ccatttgaga gatgtgtttg gcaaagctat ggggcttact
      481 gaccaagata tcgttgctct atctgggggt cacactattg gagctgcaca caaggagcgt
      541 tctggatttg agggtccctg gacctctaat cctcttattt tcgacaactc atacttcacg
      601 gagttgttga gtggtgagaa ggaaggtctc cttcagctac cttctgacaa ggctcttttg
      661 tctgaccctg tattccgccc tctcgttgac aaatatgcag cggacgaaga tgccttcttt
      721 gctgattacg ctgaggctca ccaaaagctt tccgagcttg ggtttgctga tgccctgcag
      781 ctgcctcccc tggagcgcct gaccctggac taa
//
