LOCUS       Exported                 906 bp ds-DNA     linear   SYN 31-MAY-2021
DEFINITION  Mammalian S. pyogenes Cas9 expression, human optimized.
ACCESSION   .
VERSION     .
KEYWORDS    M-SPcas
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(100..111)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gattatcgaa cttccgttgt gtgatcagtt tggcgttcag cagctgccgc caataatttt
       61 tcattttctt gacaacttct tctgagggga cgttatcact cttccctcta tttttatcgg
      121 atcttgtcaa cactttatta tcaatagaat catctttgag aaaagactgg ggcacgatat
      181 gatccacgtc gtagtcggag agccgattga tgtccagttc ctgatccacg tacatgtccc
      241 tgccgttctg caggtagtac aggtagagct tctcattctg aagctgggtg ttttcaactg
      301 ggtgttcctt aaggatttgg gaccccagtt cttttatacc ctcttcaatc ctcttcatcc
      361 tttccctact gttcttctgt cccttctggg tagtttggtt ctctcgggcc atctcgataa
      421 cgatattctc gggcttatgc cttcccatta ctttgacgag ttcatccacg accttaacgg
      481 tctgcagtat tccctttttg atagctgggc tacctgcaag attagcgatg tgctcgtgaa
      541 gactgtcccc ctggccagaa acttgtgctt tctggatgtc ctccttaaag gtgagagagt
      601 catcatggat caactgcatg aagttccggt tggcaaatcc atcggactta agaaaatcca
      661 ggattgtctt tccactctgc ttgtctcgga tcccattgat cagttttctt gacagccgcc
      721 cccatcctgt atatcggcgc ctcttgagct gtttcatgac tttgtcgtcg aagagatgag
      781 cgtaagtttt caagcgttct tcaatcatct ccctatcttc aaacaacgta agggtgagga
      841 caatgtcctc aagaatgtcc tcgttctcct cattgtccag gaagtccttg tctttaatga
      901 ttttca
//
