LOCUS       Exported               10753 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral expression vector. Contains an altered Kozak sequence 
            and/or upstream open reading frames to modulate expression at the 
            level of translation..
ACCESSION   .
VERSION     .
KEYWORDS    pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Ferreira JP, Overton KW, Wang CL
  TITLE     Tuning gene expression with synthetic upstream open reading frames.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 
            10.1073/pnas.1305590110. Epub 2013 Jun 24.
  PUBMED    23798422
REFERENCE   2  (bases 1 to 10753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             61..771
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(100..121)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(361..380)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     708..729
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    807..1380
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(974..991)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1201..1220
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1402..1411
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1408..2118
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1553..1571)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1821..1840
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2131..2719
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2184..2204)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2602..2613)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2864..2897
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     LTR             2917..3097
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3122..3140)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3123..3140)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3430..3449)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3663..3682)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3782..3804
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3842..3860)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3928..4032
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4033..4893
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4251..4270)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5064..5652
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5553..5572
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5806..5823
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5893..5913)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5898..6227
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6078..6213
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6140..6159
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          7361..7426
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7556..7576
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8001..8135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8038..8057)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8092..8111
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8304..8937
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8984..9109
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9606..9839
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10024..10068
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10335..10452
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10508..10719
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tcgagtaact aactaaacca tgggttgata aaccatgggt tgattaccat gggcgaattc
       61 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      121 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      181 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      241 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      301 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      361 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      421 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      481 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      541 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      601 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      661 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      721 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gttcgaataa
      781 gcatgcatct agggcggcca attccgcccc tctccctccc ccccccctaa cgttactggc
      841 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgtgattttc caccatattg
      901 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
      961 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     1021 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     1081 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     1141 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     1201 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     1261 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     1321 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     1381 gcttgccaca acccgggatc cgccaccatg gtgagcaagg gcgaggagga taacatggcc
     1441 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     1501 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     1561 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     1621 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     1681 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     1741 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     1801 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     1861 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     1921 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     1981 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     2041 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     2101 gacgagctgt acaagtaagc ggccgccgat aatcaacctc tggattacaa aatttgtgaa
     2161 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2221 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2281 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2341 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2401 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2461 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     2521 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     2581 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     2641 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     2701 ctttgggccg cctccccgca tcgggcaatt cgagctcggt acctttaaga ccaatgactt
     2761 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     2821 ttcactccca acgaagacaa gatgggatca attcaccatg ggaataactt cgtatagcat
     2881 acattatacg aagttatgct gctttttgct tgtactgggt ctctctggtt agaccagatc
     2941 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     3001 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     3061 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
     3121 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     3181 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     3241 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     3301 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     3361 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     3421 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     3481 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     3541 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     3601 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     3661 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     3721 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3781 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     3841 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3901 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3961 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4021 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4081 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4141 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4201 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4261 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4321 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4381 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4441 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4501 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4561 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4621 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4681 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4741 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4801 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4861 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4921 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4981 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5041 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5101 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5161 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     5221 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5281 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5341 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5401 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     5461 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5521 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5581 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5641 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5701 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5761 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5821 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     5881 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5941 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6001 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6061 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6121 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6181 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca
     6241 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     6301 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     6361 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     6421 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     6481 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     6541 ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     6601 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     6661 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
     6721 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     6781 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
     6841 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
     6901 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
     6961 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
     7021 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
     7081 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
     7141 tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg
     7201 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
     7261 aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac
     7321 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     7381 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     7441 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     7501 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     7561 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     7621 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     7681 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
     7741 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
     7801 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
     7861 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
     7921 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     7981 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     8041 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8101 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     8161 atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat
     8221 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     8281 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     8341 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     8401 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     8461 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     8521 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     8581 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     8641 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     8701 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     8761 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8821 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8881 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     8941 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     9001 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     9061 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     9121 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     9181 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     9241 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     9301 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     9361 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     9421 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     9481 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     9541 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     9601 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     9661 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     9721 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     9781 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     9841 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     9901 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     9961 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    10021 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    10081 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    10141 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    10201 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    10261 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
    10321 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    10381 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    10441 attcaaaatt ttccgatcac gagactagcc tcgacaagct tgatcgatgg ctccggtgcc
    10501 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
    10561 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
    10621 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
    10681 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg tgtcgtgacg cgcatccagg
    10741 cctaagctta cgc
//
