LOCUS Exported 10724 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.. ACCESSION . VERSION . KEYWORDS pWPT-/UAA-mEGFP-IRES-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10724) AUTHORS Ferreira JP, Overton KW, Wang CL TITLE Tuning gene expression with synthetic upstream open reading frames. JOURNAL Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 10.1073/pnas.1305590110. Epub 2013 Jun 24. PUBMED 23798422 REFERENCE 2 (bases 1 to 10724) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10724 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 32..742 /codon_start=1 /product="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEGFP /note="mammalian codon-optimized" /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF VTAAGITLGMDELYK" primer_bind complement(71..92) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(332..351) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 679..700 /label=EGFP-C /note="EGFP, forward primer" misc_feature 778..1351 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(945..962) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 1172..1191 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" regulatory 1373..1382 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1379..2089 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(1524..1542) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1792..1811 /label=mCherry-F /note="mCherry, forward primer" misc_feature 2102..2690 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2155..2175) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2573..2584) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind 2835..2868 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 2888..3068 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" promoter complement(3093..3111) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(3094..3111) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(3401..3420) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(3634..3653) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3753..3775 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3813..3831) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3899..4003 /gene="bla" /label=AmpR promoter CDS 4004..4864 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4222..4241) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5035..5623 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5524..5543 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5777..5794 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(5864..5884) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5869..6198 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6049..6184 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6111..6130 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 7332..7397 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 7527..7547 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 7972..8106 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8009..8028) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8063..8082 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 8275..8908 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 8955..9080 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9577..9810 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9995..10039 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 10306..10423 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 10479..10690 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" ORIGIN 1 tcgagtaact aactaataaa tgggcgaatt cagcaagggc gaggagctgt tcaccggggt 61 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 121 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 181 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt 241 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 301 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 361 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 421 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 481 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 541 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 601 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc 661 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 721 cggcatggac gagctgtaca agttcgaata agcatgcatc tagggcggcc aattccgccc 781 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc 841 gtttgtctat atgtgatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa 901 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat 961 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac 1021 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg 1081 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt 1141 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg 1201 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac 1261 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga 1321 cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccgggat ccgccaccat 1381 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt 1441 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg 1501 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt 1561 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc 1621 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt 1681 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg 1741 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat 1801 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc 1861 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga 1921 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa 1981 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg 2041 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgccga 2101 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 2161 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 2221 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 2281 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 2341 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 2401 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 2461 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 2521 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 2581 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 2641 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgggcaat 2701 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 2761 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatgggatc 2821 aattcaccat gggaataact tcgtatagca tacattatac gaagttatgc tgctttttgc 2881 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 2941 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 3001 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 3061 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt 3121 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct 3181 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc 3241 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa 3301 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc 3361 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc 3421 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg 3481 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt 3541 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg 3601 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 3661 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc 3721 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc 3781 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt 3841 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 3901 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 3961 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 4021 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 4081 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 4141 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 4201 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 4261 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 4321 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 4381 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 4441 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 4501 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 4561 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 4621 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 4681 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 4741 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 4801 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 4861 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 4921 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 4981 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 5041 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 5101 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 5161 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 5221 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 5281 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 5341 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 5401 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa 5461 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 5521 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 5581 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 5641 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 5701 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 5761 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 5821 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta 5881 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 5941 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 6001 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 6061 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 6121 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 6181 ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat 6241 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat 6301 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac 6361 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac 6421 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca 6481 atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt 6541 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag 6601 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat 6661 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac 6721 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat 6781 atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca 6841 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc 6901 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa 6961 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg 7021 ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc 7081 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga 7141 aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt 7201 atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct 7261 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt 7321 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa 7381 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg 7441 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta 7501 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg 7561 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg 7621 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg 7681 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt 7741 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta 7801 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga 7861 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 7921 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 7981 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 8041 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 8101 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct 8161 attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt cctctgaatt 8221 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag 8281 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag 8341 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct 8401 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag 8461 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag 8521 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc 8581 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg 8641 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca 8701 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg 8761 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 8821 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 8881 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag 8941 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag 9001 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga 9061 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa 9121 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca 9181 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 9241 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat 9301 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg 9361 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg 9421 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt 9481 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag 9541 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt 9601 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca 9661 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca 9721 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt 9781 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat 9841 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg 9901 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca 9961 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt 10021 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa 10081 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc 10141 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac 10201 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga 10261 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg 10321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 10381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttccgatca cgagactagc 10441 ctcgacaagc ttgatcgatg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 10501 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg 10561 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 10621 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 10681 cagaacacag gtgtcgtgac gcgcatccag gcctaagctt acgc //