LOCUS       Exported                 971 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    AmCyan-P2A-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Potorac I, Rivero-Muller A, Trehan A, Kielbus M, Jozwiak K, Pralong 
            F, Hafidi A, Thiry A, Menage JJ, Huhtaniemi IT, Beckers A, Daly AF
  TITLE     A vital region for human glycoprotein hormone trafficking revealed 
            by an LHB mutation.
  JOURNAL   J Endocrinol. 2016 Sep 21. pii: JOE-16-0384.
  PUBMED    27656125
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             53..73
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             77..97
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             101..121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             137..823
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPHAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKMTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHAVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPF"
     CDS             845..901
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 aaccgtcaga tcccgtctcc gtacgatggc cacaaccatg gtgcacgtgg atccaaaaaa
       61 gaagagaaag gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt
      121 acacgtgtcc acgcgtatgg ctctttcaaa caagtttatc ggagatgaca tgaaaatgac
      181 ctaccatatg gatggctgtg tcaatgggca ttactttacc gtcaaaggtg aaggcagcgg
      241 gaagccatac gaagggacgc agacctcgac ttttaaagtc accatggcca acggtgggcc
      301 ccatgcattc tcctttgaca tactatctac agtgttcatg tatggaaatc gatgctttac
      361 tgcgtatcct accagtatgc ccgactattt caaacaagca tttcctgacg gaatgtcata
      421 tgaaaggact tttacctatg aagatggagg agttgctaca gccagttggg aaataagcct
      481 taaaggcaac tgctttgagc acaaatccac gtttcatgga gtgaactttc ctgctgatgg
      541 acctgtgatg gcgaagatga caactggttg ggacccatct tttgagaaaa tgactgtctg
      601 cgatggaata ttgaagggtg atgtcaccgc gttcctcatg ctgcaaggag gtggcaatta
      661 cagatgccaa ttccacactt cttacaagac aaaaaaaccg gtgacgatgc caccaaacca
      721 tgcggtggaa catcgcattg cgaggaccga ccttgacaaa ggtggcaaca gtgttcagct
      781 gacggagcac gctgttgcac atataacctc tgttgtccct ttcgcgcgcg agacgggaag
      841 cggagctact aacttcagcc tgctgaagca ggctggcgac gtggaggaga accctggacc
      901 tggtctccgg ccgatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
      961 catgcgcttc a
//
