LOCUS Exported 5337 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION expresses EGFP-Rab6B in mammalian cells. ACCESSION . VERSION . KEYWORDS EGFP-Rab6B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5337) AUTHORS Rzomp KA, Scholtes LD, Briggs BJ, Whittaker GR, Scidmore MA TITLE Rab GTPases are recruited to chlamydial inclusions in both a species-dependent and species-independent manner. JOURNAL Infect Immun. 2003 Oct;71(10):5855-70. PUBMED 14500507 REFERENCE 2 (bases 1 to 5337) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5337 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 786..907 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(823..842) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 877..896 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(914..1369) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1051..1072) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1263..1282 /label=F1ori-R /note="F1 origin, reverse primer" promoter 1396..1500 /gene="bla" /label=AmpR promoter promoter 1502..1859 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(1525..1545) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 1710..1845 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1772..1791 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 1894..2688 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(1948..1967) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 2558..2577 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(2876..2895) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 2920..2967 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3296..3884 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3785..3804 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 4059..4362 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4363..4566 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4517..4537 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 4605..4614 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4611..5327 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 4611..5327 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(4656..4677) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(4917..4936) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5264..5285 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 gatccaccat gtccgcaggg ggagattttg ggaatccact gagaaaattc aagttggtgt 61 tcttggggga gcagagcgtc gggaagacgt ctctgattac gaggttcatg tacgacagct 121 tcgacaacac ataccaggca accattggga ttgacttctt gtcaaaaacc atgtacttgg 181 aggaccgcac ggtgcgactg cagctctggg acacagctgg tcaggagagg ttccgcagcc 241 tgatccccag ctacatccgg gactccacgg tggctgtggt ggtgtacgac atcacaaatc 301 tcaactcctt ccaacagacc tctaagtgga tcgacgacgt caggacagag aggggcagtg 361 atgttatcat catgctggtg ggcaacaaga cggacctggc tgataagagg cagataacca 421 tcgaggaggg ggagcagcgc gccaaagaac tgagcgtcat gttcattgag accagtgcga 481 agactggcta caacgtgaag cagctttttc gacgtgtggc gtcggctcta cccggaatgg 541 agaatgtcca ggagaaaagc aaagaaggga tgatcgacat caagctggac aaaccccagg 601 agcccccggc cagcgagggc ggctgctcct gctaactcga cggtaccgcg ggcccgggat 661 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg 721 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 781 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 841 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 901 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 961 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 1021 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 1081 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 1141 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 1201 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 1261 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 1321 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact 1381 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 1441 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 1501 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 1561 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 1621 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 1681 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 1741 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 1801 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag 1861 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 1921 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 1981 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 2041 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 2101 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 2161 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 2221 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 2281 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 2341 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 2401 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc 2461 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 2521 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 2581 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 2641 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 2701 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 2761 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 2821 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa 2881 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata 2941 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 3001 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 3061 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 3121 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt 3181 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 3241 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 3301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 3361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 3421 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 3481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 3541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 3601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 3661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 3721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 3781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 3841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 3901 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 3961 cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt 4021 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 4081 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 4141 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 4201 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 4261 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 4321 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 4381 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 4441 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 4501 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 4561 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagca 4621 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 4681 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 4741 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 4801 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 4861 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 4921 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 4981 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 5041 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 5101 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 5161 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 5221 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 5281 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggactca //