LOCUS       Exported                 996 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  expresses human p53 deltaN and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pIRES2-mCherry-p53 deltaN
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Lin SC, Karoly ED, Taatjes DJ
  TITLE     The human DeltaNp53 isoform triggers metabolic and gene expression 
            changes that activate mTOR and alter mitochondrial function.
  JOURNAL   Aging Cell. 2013 Oct;12(5):863-72. doi: 10.1111/acel.12108. Epub 
            2013 Jun 25.
  PUBMED    23734707
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             143..853
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKELMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFDWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(288..306)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     556..575
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca
       61 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      121 ctttgaaaaa cacgatgata atatggtgag caagggcgag gaggataaca tggccatcat
      181 caaggagtta atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      241 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      301 gaccaagggt ggccccctgc ccttcgactg ggacatcctg tcccctcagt tcatgtacgg
      361 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      421 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      481 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      541 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      601 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
      661 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      721 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      781 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      841 gctgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag
      901 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      961 gcaattgttg ttgttaactt gtttattgca gcttat
//
