LOCUS Exported 2907 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Negative feedback plasmid from oscillator without luxR. ACCESSION . VERSION . KEYWORDS pTD103aiiA(Cm) -R SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2907) AUTHORS Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J TITLE A sensing array of radically coupled genetic 'biopixels'. JOURNAL Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. PUBMED 22178928 REFERENCE 2 (bases 1 to 2907) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2907 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 197..208 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" terminator 1018..1104 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind complement(1139..1156) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1273..1818) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(1353..1372) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1932..2026 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2047..2706) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2621..2640 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(2707..2809) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" primer_bind 2875..2893 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 ctcgagacct attgtttgtc gcaagttttg cgtgttatat atcattaaaa cggtaatgga 61 ttgacatttg attctaataa attggatttt tgtcacacta ttgtatcgct gggaatacaa 121 ttacttaaca taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata 181 gtcgaatgaa ttcattaaag aggagaaagg taccatgaca gtaaagaaac tttatttcat 241 cccagcaggt cgttgcatgt tggatcattc gtctgttaac agtgcgttaa caccggggaa 301 actattaaac ttgccggtgt ggtgttatct tttggagacg gaagaaggtc ctattttagt 361 agacacaggt atgccagaaa gtgcagttaa taatgaaggg ctttttaacg gtacatttgt 421 tgaaggacag atcttaccga aaatgactga agaagataga atcgtgaata tattaaagcg 481 tgtagggtat gagccggacg accttttata tattattagt tctcacttac attttgatca 541 tgcaggagga aacggtgctt ttacaaatac accaattatt gtgcagcgaa cggaatatga 601 ggcagcactt catagagaag aatatatgaa agaatgtata ttaccgcatt tgaactacaa 661 aattattgaa ggggattatg aagtggtacc aggtgttcaa ttattgtata cgccaggtca 721 ttctccaggc catcagtcgc tattcattga gacggagcaa tccggttcag ttttattaac 781 gattgatgca tcgtacacga aagagaattt tgaagatgaa gtgccgttcg caggatttga 841 tccagaatta gctttatctt caattaaacg tttaaaagaa gttgtgaaaa aagagaaacc 901 aattattttc tttggtcatg atatagagca ggaaaagagt tgtagagtgt tcccggaata 961 tatagcagcg aacgacgaaa attacgccct tgcagcgtaa acgcgtgcta gaggcatcaa 1021 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 1081 aacgctctcc tgagtaggac aaatccgccg ccctagacct agggatatat tccgcttcct 1141 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac 1201 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc 1261 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc 1321 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg 1381 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg 1441 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc 1501 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc 1561 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt 1621 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt 1681 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga 1741 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc 1801 agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca 1861 gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtta 1921 ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa 1981 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat 2041 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct 2101 gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac 2161 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat 2221 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa 2281 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc 2341 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga 2401 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac 2461 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag 2521 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc 2581 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc 2641 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt 2701 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag 2761 tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt 2821 tcgccagata tcgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg 2881 cgtatcacga ggccctttcg tcttcac //