LOCUS       Exported                5793 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lux-regulated expression of NDH-2 enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    pZSm45 ndhII
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J
  TITLE     A sensing array of radically coupled genetic 'biopixels'.
  JOURNAL   Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722.
  PUBMED    22178928
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     RBS             962..973
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2141..2152
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      2335..2421
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      2920..3142
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3190..4140
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFKIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      4671..4765
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4777..5568)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttaattttta aagtatgggc aatcaattgc tcctgttaaa attgctttag aaatactttg
       61 gcagcggttt gttgtattga gtttcatttg cgcattggtt aaatggaaag tgacagtacg
      121 ctcactgcag cctaatattt ttgaaatatc ccaagagctt tttccttcgc atgcccacgc
      181 taaacattct ttttctcttt tggttaaatc gttgtttgat ttattatttg ctatatttat
      241 ttttcgataa ttatcaacta gagaaggaac aattaatggt atgttcatac acgcatgtaa
      301 aaataaacta tctatatagt tgtctttttc tgaatgtgca aaactaagca ttccgaagcc
      361 attgttagcc gtatgaatag ggaaactaaa cccagtgata agacctgatg ttttcgcttc
      421 tttaattaca tttggagatt ttttatttac agcattgttt tcaaatatat tccaattaat
      481 tggtgaatga ttggagttag aataatctac tataggatca tattttatta aattagcgtc
      541 atcataatat tgcctccatt ttttagggta attatctaga attgaaatat cagatttaac
      601 catagaatga ggataaatga tcgcgagtaa ataatattca caatgtacca ttttagtcat
      661 atcagataag cattgattaa tatcattatt gcttctacaa gctttaattt tattaattat
      721 tctgtatgtg tcgtcggcat ttatgttttt catacccatc tctttatcct tacctattgt
      781 ttgtcgcaag ttttgcgtgt tatatatcat taaaacggta atggattgac atttgattct
      841 aataaattgg atttttgtca cactattgta tcgctgggaa tacaattact taacataagc
      901 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atgaattcat
      961 taaagaggag aaaggtacct tgactacgcc attgaaaaag attgtgattg tcggcggcgg
     1021 tgctggtggg ctggaaatgg caacacagct ggggcataag ctgggacgca agaaaaaagc
     1081 caaaattacg ctggtcgatc gtaaccacag ccacctgtgg aaaccgctgc tgcacgaagt
     1141 ggcgactggc tcgcttgatg aaggcgtcga tgcgttgagc tatctggccc atgcgcgcaa
     1201 tcatggtttc cagttccagc tgggttccgt cattgatatt gatcgtgaag cgaaaacaat
     1261 cactattgca gaactgcgcg acgagaaagg tgaactgctg gttccggaac gtaaaatcgc
     1321 ctatgacacc ctggtaatgg cgctgggtag cacctctaac gatttcaata cgccaggtgt
     1381 caaagagaac tgcattttcc tcgataaccc gcaccaggcg cgtcgcttcc accaggagat
     1441 gctgaatttg ttcctgaaat actccgccaa cctgggcgcg aatggcaaag tgaacattgc
     1501 gattgtcggc ggcggcgcga cgggtgtaga actctccgct gaattgcaca acgcggtcaa
     1561 gcaactgcac agctacggtt acaaaggcct gaccaacgaa gccctgaacg taacgctggt
     1621 agaagcggga gaacgtattt tgcctgcgtt accgccacgt atctctgctg cggcccacaa
     1681 cgagctaacg aaacttggcg ttcgcgtgct gacgcaaacc atggtcacca gtgctgatga
     1741 aggcggcctg cacactaaag atggcgaata tattgaggct gatctgatgg tatgggcagc
     1801 cgggatcaaa gcgccagact tcctgaaaga tatcggtggt cttgaaacta accgtatcaa
     1861 ccagctggtg gtggaaccga cgctgcaaac cacccgcgat ccagacattt acgctattgg
     1921 cgactgcgcg tcatgcccgc gtccggaagg gggctttgtt ccgccgcgtg ctcaggctgc
     1981 acaccagatg gcgacttgcg caatgaacaa cattctggcg cagatgaacg gtaagccgct
     2041 gaaaaattat cagtataaag atcatggttc gctggtatcg ctgtcgaact tctccaccgt
     2101 cggtagcctg atgggtaacc tgacgcgcgg ctcaatgatg attgaaggac gaattgcgcg
     2161 ctttgtatat atctcgctat accgaatgca tcagattgcg ctgcatggtt actttaaaac
     2221 cggattaatg atgctggtgg ggagtattaa ccgcgttatc cgtccgcgtt tgaagttgca
     2281 tgcagcgaac gacgaaaatt acgcccttgc agcgtaaacg cgtgctagag gcatcaaata
     2341 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2401 gctctcctga gtaggacaaa tccgccgccc tagacctagg gacagtaaga cgggtaagcc
     2461 tgttgatgat accgctgcct tactgggtgc attagccagt ctgaatgacc tgtcacggga
     2521 taatccgaag tggtcagact ggaaaatcag agggcaggaa ctgctgaaca gcaaaaagtc
     2581 agatagcacc acatagcaga cccgccataa aacgccctga gaagcccgtg acgggctttt
     2641 cttgtattat gggtagtttc cttgcatgaa tccataaaag gcgcctgtag tgccatttac
     2701 ccccattcac tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa agacagcgac
     2761 tcaggtgcct gatggtcgga gacaaaagga atattcagcg atttgcccga gcttgcgagg
     2821 gtgctactta agcctttagg gttttaaggt ctgttttgta gaggagcaaa cagcgtttgc
     2881 gacatccttt tgtaatactg cggaactgac taaagtagtg agttatacac agggctggga
     2941 tctattcttt ttatcttttt ttattctttc tttattctat aaattataac cacttgaata
     3001 taaacaaaaa aaacacacaa aggtctagcg gaatttacag agggtctagc agaatttaca
     3061 agttttccag caaaggtcta gcagaattta cagataccca caactcaaag gaaaaggaca
     3121 agtaattatc attgactagc ccatctcaat tggtatagtg attaaaatca cctagaccaa
     3181 ttgagatgta tgtctgaatt agttgttttc aaagcaaatg aactagcgat tagtcgctat
     3241 gacttaacgg agcatgaaac caagctaatt ttatgctgtg tggcactact caaccccacg
     3301 attgaaaacc ctacaaggaa agaacggacg gtatcgttca cttataacca atacgctcag
     3361 atgatgaaca tcagtaggga aaatgcttat ggtgtattag ctaaagcaac cagagagctg
     3421 atgacgagaa ctgtggaaat caggaatcct ttggttaaag gctttaagat tttccagtgg
     3481 acaaactatg ccaagttctc aagcgaaaaa ttagaattag tttttagtga agagatattg
     3541 ccttatcttt tccagttaaa aaaattcata aaatataatc tggaacatgt taagtctttt
     3601 gaaaacaaat actctatgag gatttatgag tggttattaa aagaactaac acaaaagaaa
     3661 actcacaagg caaatataga gattagcctt gatgaattta agttcatgtt aatgcttgaa
     3721 aataactacc atgagtttaa aaggcttaac caatgggttt tgaaaccaat aagtaaagat
     3781 ttaaacactt acagcaatat gaaattggtg gttgataagc gaggccgccc gactgatacg
     3841 ttgattttcc aagttgaact agatagacaa atggatctcg taaccgaact tgagaacaac
     3901 cagataaaaa tgaatggtga caaaatacca acaaccatta catcagattc ctacctacgt
     3961 aacggactaa gaaaaacact acacgatgct ttaactgcaa aaattcagct caccagtttt
     4021 gaggcaaaat ttttgagtga catgcaaagt aagcatgatc tcaatggttc gttctcatgg
     4081 ctcacgcaaa aacaacgaac cacactagag aacatactgg ctaaatacgg aaggatctga
     4141 ggttcttatg gctcttgtat ctatcagtga agcatcaaga ctaacaaaca aaagtagaac
     4201 aactgttcac cgttagatat caaagggaaa actgtccata tgcacagatg aaaacggtgt
     4261 aaaaaagata gatacatcag agcttttacg agtttttggt gcatttaaag ctgttcacca
     4321 tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca cctaatagaa caggtgaaac
     4381 cagtaaaaca aagcaactag aacatgaaat tgaacacctg agacaacttg ttacagctca
     4441 acagtcacac atagacagcc tgaaacaggc gatgctgctt atcgaatcaa agctgccgac
     4501 aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca tggcaattct ggaagaaata
     4561 gcgctttcag ccggcaaacc ggctgaagcc ggatctgcga ttctgataac aaactagcaa
     4621 caccagaaca gcccgtttgc gggcagcaaa acccgtacac tagtgcttgg attctcacca
     4681 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     4741 attactggat ctatcaacag gagtccaagc gagctcttat ttgccgacta ccttggtgat
     4801 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     4861 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     4921 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     4981 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     5041 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     5101 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     5161 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     5221 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     5281 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     5341 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     5401 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     5461 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     5521 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatga tgtttaactt
     5581 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     5641 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     5701 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     5761 ggttctggac cagttgcgtg agcgcatgac gtc
//
