LOCUS       Exported                 893 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    BRCA1 (1-304)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE
  TITLE     A UbcH5/ubiquitin noncovalent complex is required for processive 
            BRCA1-directed ubiquitination.
  JOURNAL   Mol Cell. 2006 Mar 17. 21(6):873-80.
  PUBMED    16543155
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggatttat ctgctcttcg cgttgaagaa gtacaaaatg tcattaatgc tatgcagaaa
      121 atcttagagt gtcccatctg tctggagttg atcaaggaac ctgtctccac aaagtgtgac
      181 cacatatttt gcaaattttg catgctgaaa cttctcaacc agaagaaagg gccttcacag
      241 tgtcctttat gtaagaatga tataaccaaa aggagcctac aagaaagtac gagatttagt
      301 caacttgttg aagagctatt gaaaatcatt tgtgcttttc agcttgacac aggtttggag
      361 tatgcaaaca gctataattt tgcaaaaaag gaaaataact ctcctgaaca tctaaaagat
      421 gaagtttcta tcatccaaag tatgggctac agaaaccgtg ccaaaagact tctacagagt
      481 gaacccgaaa atccttcctt gcaggaaacc agtctcagtg tccaactctc taaccttgga
      541 actgtgagaa ctctgaggac aaagcagcgg atacaacctc aaaagacgtc tgtctacatt
      601 gaattgggat ctgattcttc tgaagatacc gttaataagg caacttattg cagtgtggga
      661 gatcaagaat tgttacaaat cacccctcag gaaccaggga tgaaatcagt ttggattctg
      721 caaaaagctg cttgtgaatt ttctgaacgg atgtacaaat actgaaatca tcaccgtaat
      781 atgatttgaa ccccctgaaa acgtgcatga aagatccaaa aagttcggga tctgtttcaa
      841 actgcatggg ggcctgggac aaaactgccg ctcttacgca tgaacgcatt tcc
//
