LOCUS       Exported                 666 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Mammalian expression of humanized ChR2 with H134R mutation fused to 
            mCherry for optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3.1/hChR2(H134R)-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, 
            Bamberg E, Nagel G, Gottschalk A, Deisseroth K
  TITLE     Multimodal fast optical interrogation of neural circuitry.
  JOURNAL   Nature. 2007 Apr 5. 446(7136):633-9.
  PUBMED    17410168
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      83..92
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 ctatagggag acccaagctg gctagttaag cttggtaccg agctcggatc cactagtcca
       61 gtgtggtgga attgcccttg ctgccaccat ggactatggc ggcgctttgt ctgccgtcgg
      121 acgcgaactt ttgttcgtta ctaatcctgt ggtggtgaac gggtccgtcc tggtccctga
      181 ggatcaatgt tactgtgccg gatggattga atctcgcggc acgaacggcg ctcagaccgc
      241 gtcaaatgtc ctgcagtggc ttgcagcagg attcagcatt ttgctgctga tgttctatgc
      301 ctaccaaacc tggaaatcta catgcggctg ggaggagatc tatgtgtgcg ccattgaaat
      361 ggttaaggtg attctcgagt tcttttttga gtttaagaat ccctctatgc tctaccttgc
      421 cacaggacac cgggtgcagt ggctgcgcta tgcagagtgg ctgctcactt gtcctgtcat
      481 ccttatccgc ctgagcaacc tcaccggcct gagcaacgac tacagcagga gaaccatggg
      541 actccttgtc tcagacatcg ggactatcgt gtggggggct accagcgcca tggcaaccgg
      601 ctatgttaaa gtcatcttct tttgtcttgg attgtgctat ggcgcgaaca cattttttca
      661 cgccgc
//
