LOCUS Exported 936 bp ds-DNA linear SYN 03-JUN-2021 DEFINITION FX cloning insect cell expression vector. ACCESSION . VERSION . KEYWORDS pFBXC3H SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 936) TITLE Weinert et al, Grutter lab JOURNAL Unpublished REFERENCE 2 (bases 1 to 936) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 3, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..936 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(85..115) /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" protein_bind complement(140..264) /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS complement(283..303) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(307..327) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 354..377 /codon_start=1 /product="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" /label=HRV 3C site /translation="LEVLFQGP" CDS 378..407 /codon_start=1 /product="10xHis affinity tag" /label=10xHis /translation="HHHHHHHHHH" polyA_signal 564..698 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(601..620) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 655..674 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" mobile_element 727..892 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg 61 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg 121 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt 181 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg 241 ttcagctttt ttgtacaaac ttgtgatatc gaattcgcta gctacctttc tcttcttttt 301 tggatctacc tttctcttct tttttgggga tccactagtt cagctcttct gcattagaag 361 ttttgtttca aggtccacac catcatcatc accatcatca tcatcattaa taatctagag 421 cctgcagtct cgacaagctt gtcgagaagt actagaggat cataatcagc cataccacat 481 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata 541 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa 601 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 661 tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagga 721 gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt aattttcgta 781 ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct 841 taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca 901 tttttcttcc tgttatgttt ttaatcaaac atcctg //