LOCUS Exported 8609 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Destination Vector for TALEColor-mVenus. ACCESSION . VERSION . KEYWORDS pTH-NI(A)-mVenus SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8609) AUTHORS Ma H, Reyes-Gutierrez P, Pederson T TITLE Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors. JOURNAL Proc Natl Acad Sci U S A. 2013 Dec 9. PUBMED 24324157 REFERENCE 2 (bases 1 to 8609) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8609 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(43..62) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 234..613 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 614..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 768..788 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 819..837 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 840..858 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 868..892 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 1004..1013 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1013..1078 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 1085..1105 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" promoter 1574..1604 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1658..2317 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1724..1743) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 2659..2964 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 2843..2862 /label=ccdB-fwd /note="ccdB gene, forward primer" CDS 3283..4002 /codon_start=1 /product="Venus YFP with monomerizing A206K mutation (Nagai et al., 2002; Kremers et al., 2006)" /label=mVenus /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3328..3349) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3589..3608) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3936..3957 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(4034..4051) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4040..4264 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4310..4738 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4397..4416) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4607..4628 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4747..4767) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4752..5081 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4932..5067 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4994..5013 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5130..6155 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" polyA_signal 6285..6406 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6322..6341) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6376..6395 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6455..6471) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6455..6471) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6468..6490) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6479..6495 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6503..6533) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6548..6569 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6686..6703) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6857..7442) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6937..6956) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7613..8473) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8236..8255 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8474..8578) /gene="bla" /label=AmpR promoter ORIGIN 1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc 61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc 121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattccatga agaatctgct 181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg 241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga 661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca 721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg 781 taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct 841 ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag 901 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctggcgcg 961 ccggttctgg atccggttct caattgggtt ctaccggtta ggggccacca tggactataa 1021 ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg acgataagat 1081 ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg tagatttgag 1141 aactttggga tattcacagc agcagcagga aaagatcaag cccaaagtga ggtcgacagt 1201 cgcgcagcat cacgaagcgc tggtgggtca tgggtttaca catgcccaca tcgtagcctt 1261 gtcgcagcac cctgcagccc ttggcacggt cgccgtcaag taccaggaca tgattgcggc 1321 gttgccggaa gccacacatg aggcgatcgt cggtgtgggg aaacagtgga gcggagcccg 1381 agcgcttgag gccctgttga cggtcgcggg agagctgaga gggcctcccc ttcagctgga 1441 cacgggccag ttgctgaaga tcgcgaagcg gggaggagtc acggcggtcg aggcggtgca 1501 cgcgtggcgc aatgcgctca cgggagcacc cctcaacctg acagagaccg cggccgcatt 1561 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta 1621 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata 1681 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt 1741 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt 1801 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa 1861 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt 1921 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga 1981 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg 2041 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa 2101 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc 2161 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc 2221 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt 2281 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa 2341 agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga 2401 tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca 2461 gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg 2521 taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa 2581 atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg 2641 agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat 2701 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc 2761 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg 2821 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc 2881 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt 2941 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt 3001 cgacggtctc gactcacgcc tgagcaggta gtggctattg catccaacat cgggggcaga 3061 cccgcactgg agtcaatcgt ggcccagctt tcgaggccgg accccgcgct ggccgcactc 3121 actaatgatc atcttgtagc gctggcctgc ctcggcggac gacccgcctt ggatgcggtg 3181 aagaaggggc tcccgcacgc gcctgcattg attaagcgga ccaacagaag gattcccgag 3241 aggacatcac atcgagtggc aggcctgcag ggaagtggaa gtatggtgag caagggcgag 3301 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 3361 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 3421 ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc 3481 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag 3541 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 3601 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 3661 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 3721 aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc 3781 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac 3841 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc 3901 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 3961 gccgccggga tcactctcgg catggacgag ctgtacaagt agtctagagg gcccgtttaa 4021 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 4081 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 4141 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 4201 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 4261 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt 4321 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 4381 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 4441 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 4501 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 4561 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 4621 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 4681 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa 4741 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 4801 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 4861 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 4921 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 4981 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 5041 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 5101 tatccatttt cggatctgat cagcacgtga tgaaaaagcc tgaactcacc gcgacgtctg 5161 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg 5221 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa 5281 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg 5341 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca 5401 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg 5461 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga 5521 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca 5581 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca 5641 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag 5701 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca 5761 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca 5821 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc 5881 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc 5941 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg 6001 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc 6061 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc 6121 gacgccccag cactcgtccg agggcaaagg aatagcacgt gctacgagat ttcgattcca 6181 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 6241 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 6301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 6361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 6421 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 6481 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 6541 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 6601 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 6661 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 6721 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 6781 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 6841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 6901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 6961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 7321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7381 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 7441 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 7501 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 7561 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 7621 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 7681 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 7741 caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag 7801 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 7861 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 7921 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 7981 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 8041 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 8101 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 8161 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 8221 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 8281 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 8341 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 8401 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 8461 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 8521 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 8581 catttccccg aaaagtgcca cctgacgtc //