LOCUS Exported 6336 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION TALEGreen-Telomere for In Vitro Translation. ACCESSION . VERSION . KEYWORDS pT7CFE1-TelR15-sfGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6336) AUTHORS Ma H, Reyes-Gutierrez P, Pederson T TITLE Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors. JOURNAL Proc Natl Acad Sci U S A. 2013 Dec 9. PUBMED 24324157 REFERENCE 2 (bases 1 to 6336) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6336 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 1..20 /label=T7 /note="T7 promoter, forward primer" promoter 1..19 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 70..531 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(126..143) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" CDS 2582..3295 /codon_start=1 /product="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /label=superfolder GFP /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" CDS 3320..3337 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(3395..3413) /label=T7 Term /note="T7 terminator, reverse primer" terminator 3409..3456 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind complement(3485..3502) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3656..4244) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3736..3755) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4415..5275) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5038..5057 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5276..5380) /gene="bla" /label=AmpR promoter primer_bind 5448..5466 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(5504..5526) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 5626..5645 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin complement(5714..6169) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5851..5872) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6063..6082 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6299..6321 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6313..6330 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 6314..6330 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 taatacgact cactataggg cgaattaatt ccggttattt tccaccatat tgccgtcttt 61 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct 121 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct 181 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc 241 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc 301 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctcac 361 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc 421 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta 481 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac 541 cacccatatg ggatccggta tccacggagt cccagcagcc gtagatttga gaactttggg 601 atattcacag cagcagcagg aaaagatcaa gcccaaagtg aggtcgacag tcgcgcagca 661 tcacgaagcg ctggtgggtc atgggtttac acatgcccac atcgtagcct tgtcgcagca 721 ccctgcagcc cttggcacgg tcgccgtcaa gtaccaggac atgattgcgg cgttgccgga 781 agccacacat gaggcgatcg tcggtgtggg gaaacagtgg agcggagccc gagcgcttga 841 ggccctgttg acggtcgcgg gagagctgag agggcctccc cttcagctgg acacgggcca 901 gttgctgaag atcgcgaagc ggggaggagt cacggcggtc gaggcggtgc acgcgtggcg 961 caatgcgctc acgggagcac ccctcaacct gaccccagag caggtcgtgg caattgcgag 1021 caacatcggg ggaaagcagg cactcgaaac cgtccagagg ttgctgcctg tgctgtgcca 1081 agcgcacgga cttacgccag agcaggtcgt ggcaattgcg agcaacatcg ggggaaagca 1141 ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc caagcgcacg gactaacccc 1201 agagcaggtc gtggcaattg cgagccatga cgggggaaag caggcactcg aaaccgtcca 1261 gaggttgctg cctgtgctgt gccaagcgca cgggttgacc ccagagcagg tcgtggcaat 1321 tgcgagccat gacgggggaa agcaggcact cgaaaccgtc cagaggttgc tgcctgtgct 1381 gtgccaagcg cacggcctga ccccagagca ggtcgtggca attgcgagcc atgacggggg 1441 aaagcaggca ctcgaaaccg tccagaggtt gctgcctgtg ctgtgccaag cgcacggact 1501 gacaccagag caggtcgtgg caattgcgag caacggaggg ggaaagcagg cactcgaaac 1561 cgtccagagg ttgctgcctg tgctgtgcca agcgcacgga cttacacccg aacaagtcgt 1621 ggcaattgcg agcaacatcg ggggaaagca ggcactcgaa accgtccaga ggttgctgcc 1681 tgtgctgtgc caagcgcacg gacttacgcc agagcaggtc gtggcaattg cgagcaacat 1741 cgggggaaag caggcactcg aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca 1801 cggactaacc ccagagcagg tcgtggcaat tgcgagccat gacgggggaa agcaggcact 1861 cgaaaccgtc cagaggttgc tgcctgtgct gtgccaagcg cacgggttga ccccagagca 1921 ggtcgtggca attgcgagcc atgacggggg aaagcaggca ctcgaaaccg tccagaggtt 1981 gctgcctgtg ctgtgccaag cgcacggcct gaccccagag caggtcgtgg caattgcgag 2041 ccatgacggg ggaaagcagg cactcgaaac cgtccagagg ttgctgcctg tgctgtgcca 2101 agcgcacgga ctgacaccag agcaggtcgt ggcaattgcg agcaacggag ggggaaagca 2161 ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc caagcgcacg gcctcacccc 2221 agagcaggtc gtggcaattg cgagcaacat cgggggaaag caggcactcg aaaccgtcca 2281 gaggttgctg cctgtgctgt gccaagcgca cggactcacg cctgagcagg tagtggctat 2341 tgcatccaac atcgggggca gacccgcact ggagtcaatc gtggcccagc tttcgaggcc 2401 ggaccccgcg ctggccgcac tcactaatga tcatcttgta gcgctggcct gcctcggcgg 2461 acgacccgcc ttggatgcgg tgaagaaggg gctcccgcac gcgcctgcat tgattaagcg 2521 gaccaacaga aggattcccg agaggacatc acatcgagtg gcaggcctgc agggaagtgg 2581 aatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg aactggatgg 2641 tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg caactaatgg 2701 taaactgacg ctgaagttca tctgtactac tggtaaactg ccggtacctt ggccgactct 2761 ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc atatgaagca 2821 gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca cgatttcctt 2881 taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg ataccctggt 2941 aaaccgcatt gagctgaaag gcattgactt taaagaagac ggcaatatcc tgggccataa 3001 gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac aaaaaaatgg 3061 cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc agctggctga 3121 tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag acaatcacta 3181 tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc atatggttct 3241 gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt acaaatagtc 3301 tagagcggcc gcactcgagc accaccacca ccaccactga gatctgactg aaaaaaaaaa 3361 aaaaaaaaaa aaaaaaaaaa gtttaaacac tagtccgctg agcaataact agcataaccc 3421 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccggg 3481 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 3541 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 3601 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 3661 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3721 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3781 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3841 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3901 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3961 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 4021 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 4081 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 4141 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 4201 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 4261 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 4321 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 4381 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 4441 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 4501 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 4561 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 4621 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 4681 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 4741 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 4801 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 4861 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 4921 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 4981 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 5041 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 5101 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 5161 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 5221 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 5281 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 5341 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 5401 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 5461 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 5521 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 5581 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 5641 ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa 5701 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 5761 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 5821 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 5881 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 5941 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 6001 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 6061 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 6121 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 6181 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 6241 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 6301 cagtcacgac gttgtaaaac gacggccagt gaattg //