LOCUS Exported 3589 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pICSL13001 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3589) AUTHORS Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, Patron NJ, Marillonnet S TITLE A golden gate modular cloning toolbox for plants. JOURNAL ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. Epub 2014 Feb 20. PUBMED 24933124 REFERENCE 2 (bases 1 to 3589) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3589 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(40..58) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 126..230 /gene="bla" /label=AmpR promoter CDS 450..1241 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1412..2000 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1901..1920 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2154..2171 /label=L4440 /note="L4440 vector, forward primer" promoter 3241..3586 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 3529..3549 /label=35S promoter /note="CaMV 35S promoter, forward primer" ORIGIN 1 ccattgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt 61 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa 121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac 241 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca 301 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag 361 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg 421 gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga 481 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg 541 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc 601 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt 661 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg 721 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag 781 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc 841 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg 901 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa 961 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt 1021 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact 1081 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt 1141 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc 1201 attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac caagtttact 1261 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1321 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1381 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1441 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1501 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 1561 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1621 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1681 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1741 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1801 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1861 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1921 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1981 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 2041 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 2101 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 2161 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 2221 cgattcatta atcactctgt ggtctcagga ggaattccaa tcccacaaaa atctgagctt 2281 aacagcacag ttgctcctct cagagcagaa tcgggtattc aacaccctca tatcaactac 2341 tacgttgtgt ataacggtcc acatgccggt atatacgatg actggggttg tacaaaggcg 2401 gcaacaaacg gcgttcccgg agttgcacac aagaaatttg ccactattac agaggcaaga 2461 gcagcagctg acgcgtacac aacaagtcag caaacagaca ggttgaactt catccccaaa 2521 ggagaagctc aactcaagcc caagagcttt gctaaggccc taacaagccc accaaagcaa 2581 aaagcccact ggctcacgct aggaaccaaa aggcccagca gtgatccagc cccaaaagag 2641 atctcctttg ccccggagat tacaatggac gatttcctct atctttacga tctaggaagg 2701 aagttcgaag gtgaaggtga cgacactatg ttcaccactg ataatgagaa ggttagcctc 2761 ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc agcaagtctc 2821 atcaagacga tctacccgag taacaatctc caggagatca aataccttcc caagaaggtt 2881 aaagatgcag tcaaaagatt caggactaat tgcatcaaga acacagagaa agacatattt 2941 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg 3001 caagtaatag agattggagt ctctaaaaag gtagttccta ctgaatctaa ggccatgcat 3061 ggagtctaag attcaaatcg aggatctaac agaactcgcc gtcaagactg gcgaacagtt 3121 catacagagt cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca 3181 cgacactctg gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat 3241 tgagactttt caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat 3301 ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg 3361 cgataaagga aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc 3421 cccacccacg aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt 3481 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca 3541 agacccttcc tctatataag gaagttcatt tcatttggag aggacacgc //