LOCUS Exported 3001 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pICH45195 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3001) AUTHORS Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, Patron NJ, Marillonnet S TITLE A golden gate modular cloning toolbox for plants. JOURNAL ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. Epub 2014 Feb 20. PUBMED 24933124 REFERENCE 2 (bases 1 to 3001) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3001 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(40..58) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 126..230 /gene="bla" /label=AmpR promoter CDS 450..1241 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1412..2000 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1901..1920 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2154..2171 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt 61 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa 121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac 241 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca 301 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag 361 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg 421 gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga 481 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg 541 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat attgatttgc 601 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt 661 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg 721 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag 781 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc 841 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg 901 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa 961 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt 1021 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact 1081 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt 1141 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc 1201 attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac caagtttact 1261 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1321 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1381 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1441 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1501 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 1561 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1621 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1681 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1741 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1801 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1861 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1921 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1981 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 2041 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 2101 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 2161 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 2221 cgattcatta atcactctgt ggtctcagga ggcttaagtg ggccgtaagt gcaattgcgt 2281 tgtgctccaa aaggaacggt gccgtgggtt cctcttgttc atcagaaata tattaattag 2341 ccgtaaaacc tgaaaattca caagcatttg gattgttttc taatttaata tccattatgt 2401 gactaaaagt tctagtgatc gtacatacta catagaaaat aataacacaa aatactagtt 2461 tacatttccc aattaaaaac cattttgaat gaactctgtc tgatttaatt atacttttaa 2521 aatgtgggat gaattcaaag attatactta tattcttatt atttaagatt atcaagtgga 2581 aaaataaaaa tatgaatgtg ttaatataag gtaatagaaa tttaatcatt tttttaatct 2641 atatgtaaaa agtatttaac cgatatctac aatttgacgc ctcccaattg aaaggagcca 2701 aaagcaaccg atcaagtgca gaccagtagc catacacatt cactcctacc cttacatgag 2761 aaagataaga ttatggagtt ttctgccacg tgatcttatc ctagtggtcc aaatcgataa 2821 gggtgtcaac acctttcctt aatcctgtgg caattaacga cgttatcatg aattatggcc 2881 cctttgatca ttagggctag ttgcctctag cggttcccac tatataaaga tgacaaaacc 2941 aacagacaaa caagtaagta agagaaaaac caaaagaaga agagaaacaa caagaagaag 3001 t //