LOCUS Exported 2980 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pICSL30004 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2980) AUTHORS Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, Patron NJ, Marillonnet S TITLE A golden gate modular cloning toolbox for plants. JOURNAL ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. Epub 2014 Feb 20. PUBMED 24933124 REFERENCE 2 (bases 1 to 2980) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2980 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(160..951) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" promoter complement(1171..1275) /gene="bla" /label=AmpR promoter primer_bind 1343..1361 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" CDS 1397..2110 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELY" primer_bind complement(1442..1463) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1703..1722) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2050..2071 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(2210..2227) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2381..2969) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2461..2480) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 61 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 121 atcaatctaa agtatatatg agtaaacttg gtctgacagt tatttgccga ctaccttggt 181 gatctcgcct tttacgtaat ggacaaattc ttccaactga tctgcgcgcg aggccaagcg 241 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc 301 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac 361 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc 421 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg 481 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc 541 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag 601 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg 661 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc 721 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag 781 ccttacggtc accgtaacca gcaaatcaat atcgctgtgt ggcttcaggc cgccatccac 841 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc 901 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtttaa 961 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca 1021 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa 1081 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt 1141 caaggttctg gaccagttgc gtgagcgcat actcttcctt tttcaatatt attgaagcat 1201 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 1261 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 1321 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc cactgaagag 1381 ccactgtggt ctcaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 1441 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga 1501 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 1561 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcagtgct tcgcccgcta 1621 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca 1681 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt 1741 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 1801 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc 1861 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 1921 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct 1981 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa 2041 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 2101 cgagctgtac aanaacgcgg ctattagatc aatgtgagac cacgagtgat taatgaatcg 2161 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 2221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 2281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 2341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 2521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 2581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 2641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 2701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 2761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2821 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 2881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 2941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt //