LOCUS Exported 2962 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pICSL50004 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2962) AUTHORS Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, Patron NJ, Marillonnet S TITLE A golden gate modular cloning toolbox for plants. JOURNAL ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. Epub 2014 Feb 20. PUBMED 24933124 REFERENCE 2 (bases 1 to 2962) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2962 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(24..42) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 110..214 /gene="bla" /label=AmpR promoter CDS 434..1225 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1396..1984 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1885..1904 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2138..2155 /label=L4440 /note="L4440 vector, forward primer" CDS 2236..2946 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(2381..2399) /label=mCherry-R /note="mCherry, reverse primer" ORIGIN 1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac 241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg 301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg 361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac 421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt 481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc 541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt 601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc 661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat 721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga 781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac 841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc 901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc 961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag 1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct 1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga 1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga 1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat 1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact 2221 ctgtggtctc attcgatggt gagcaagggc gaggaggata acatggccat catcaaggag 2281 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag 2341 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag 2401 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag 2461 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc 2521 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac 2581 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc 2641 tccgacggcc ccgtaatgca gaagaaaacc atgggctggg aggcctcctc cgagcggatg 2701 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc 2761 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc 2821 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc 2881 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac 2941 aagtgagctt tgagaccacg aa //