LOCUS Exported 2970 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pICSL80012 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2970) AUTHORS Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, Patron NJ, Marillonnet S TITLE A golden gate modular cloning toolbox for plants. JOURNAL ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. Epub 2014 Feb 20. PUBMED 24933124 REFERENCE 2 (bases 1 to 2970) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2970 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(24..42) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 110..214 /gene="bla" /label=AmpR promoter CDS 434..1225 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1396..1984 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1885..1904 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2138..2155 /label=L4440 /note="L4440 vector, forward primer" CDS 2238..2954 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(2280..2301) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2541..2560) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2888..2909 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac 241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg 301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg 361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac 421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt 481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc 541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt 601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc 661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat 721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga 781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac 841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc 901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc 961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag 1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct 1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga 1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga 1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat 1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta 1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact 2221 ctgtggtctc aaggttagtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 2281 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 2341 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 2401 tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc 2461 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 2521 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 2581 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 2641 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 2701 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 2761 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 2821 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc 2881 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 2941 agctgtacaa gtgagctttg agaccacgaa //