LOCUS       Exported                 860 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  Lentiviral expression vector. Contains an altered Kozak sequence 
            and/or upstream open reading frames to modulate expression at the 
            level of translation..
ACCESSION   .
VERSION     .
KEYWORDS    pWPT-GGG/ACC-mEGFP-IRES-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Ferreira JP, Overton KW, Wang CL
  TITLE     Tuning gene expression with synthetic upstream open reading frames.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11284-9. doi: 
            10.1073/pnas.1305590110. Epub 2013 Jun 24.
  PUBMED    23798422
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             147..857
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(186..207)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(447..466)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     794..815
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
       61 aacacaggtg tcgtgacgcg catccaggcc taagcttacg ctcgagtaac taactaaggg
      121 atgggttgat taccatgggc gaattcagca agggcgagga gctgttcacc ggggtggtgc
      181 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      241 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      301 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      361 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      421 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      481 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      541 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      601 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
      661 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      721 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
      781 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      841 tggacgagct gtacaagttc
//
