LOCUS       Exported                 996 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  Destination vector for TALEN with TALEColor Backbone.
ACCESSION   .
VERSION     .
KEYWORDS    pTH-HD(C)-Fok1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      203..212
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             212..277
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             284..304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        773..803
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(923..942)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctggcgcgc cggttctgga tccggttctc
      181 aattgggttc taccggttag gggccaccat ggactataag gaccacgacg gagactacaa
      241 ggatcatgat attgattaca aagacgatga cgataagatg gccccaaaga agaagcggaa
      301 ggtcggtatc cacggagtcc cagcagccgt agatttgaga actttgggat attcacagca
      361 gcagcaggaa aagatcaagc ccaaagtgag gtcgacagtc gcgcagcatc acgaagcgct
      421 ggtgggtcat gggtttacac atgcccacat cgtagccttg tcgcagcacc ctgcagccct
      481 tggcacggtc gccgtcaagt accaggacat gattgcggcg ttgccggaag ccacacatga
      541 ggcgatcgtc ggtgtgggga aacagtggag cggagcccga gcgcttgagg ccctgttgac
      601 ggtcgcggga gagctgagag ggcctcccct tcagctggac acgggccagt tgctgaagat
      661 cgcgaagcgg ggaggagtca cggcggtcga ggcggtgcac gcgtggcgca atgcgctcac
      721 gggagcaccc ctcaacctga cagagaccgc ggccgcatta ggcaccccag gctttacact
      781 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
      841 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
      901 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
      961 gaccgttcag ctggatatta cggccttttt aaagac
//
