LOCUS       Exported                 928 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  Expression of a catalytically inactive, human codon-optimized Cas9 
            under the control of Murine Stem Cell retroVirus LTR promoter for 
            mammalian gene knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    pdCas9-humanized
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim 
            WA
  TITLE     Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific 
            Control of Gene Expression.
  JOURNAL   Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
  PUBMED    23452860
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 cctcgcggat aagtttgtcg ttttcgtcgt acttggtatt catgcgtgag tcaaggattt
       61 gggcgacatg tttagtaatc tgccgagtct ccacaagttg ccgtttaatg aatcctgctt
      121 tgtccagttc gctcagtccg cccctctcag ccttagtgag attgtcgaac ttccgctgag
      181 tgatcagctt tgcgttcaga agctggcgcc aatagttctt catctttttc acgacttcct
      241 ctgagggcac gttatctgac tttccccggt tcttgtctga gcgagtcagc accttattgt
      301 caattgagtc gtctttgagg aagctctgag ggacgatggc gtccacgtcg tagtctgaga
      361 gcctgttgat atcaagctct tggtccacat acatatcgcg tccattttga aggtagtaca
      421 gatagagctt ctcattctga agctgggtgt tttccaccgg gtgctcttta aggatctgtg
      481 acccgagctc cttaatccct tcctcaattc ttttcatccg ctccctgctg ttcttctgtc
      541 ccttctgggt agtctggttt tccctggcca tctcgatgac aatgttctct ggtttatggc
      601 gacccatcac cttgaccagc tcgtccacga ctttcacggt ctggagaata cccttcttaa
      661 tagcgggtga accagccaga tttgcgatat gctcgtgcag tgagtcacct tgtccagaca
      721 cttgggcctt ctggatgtcc tccttaaagg tcagggaatc atcatggata agttgcatga
      781 agttgcgatt agcgaatccg tcgctcttaa ggaaatcaag aatggtcttt ccgctctgtt
      841 tgtcccggat tccgttgatg agtttgcggc taaggcgtcc ccatccggta tatcttctcc
      901 gtttaagttg cttcatcact ttatcgtc
//
